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PMID: 2991200 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Bacteroides intermedius binds fibrinogen.

Journal of bacteriology ·Vol. 163 ·No. 2 ·1985-08-00 ·Pages 623-8

Lantz MS, Switalski LM, Kornman KS, Höök M

Abstract

The binding of Bacteroides intermedius VPI 8944 to human fibrinogen has been characterized. The binding is time dependent, at least partially reversible, saturable, and specific. On an average, a maximum of 3,500 fibrinogen molecules bind per bacterial cell, with a dissociation constant of 1.7 X 10(-11) M. These bacteria also exhibit a fibrinogenolytic activity which can be partially inhibited by protease inhibitors. Bacteria release fibrinogenolytic activity into the surrounding medium without loss of binding activity, but more pronounced fibrinogen breakdown occurs when 125I-labeled fibrinogen is associated with the bacteria, suggesting that fibrinogen is degraded at the cell surface. Fibrinogen binding by B. intermedius might represent a mechanism of bacterial tissue adherence.

MeSH Terms
Anaerobiosis Bacteroides/growth & development,isolation & purification,metabolism Binding, Competitive Dental Plaque/microbiology Fibrinogen/metabolism Humans Kinetics Molecular Weight Peptide Fragments/analysis Platelet Membrane Glycoproteins Protein Binding Receptors, Cell Surface/metabolism
Chemicals
Peptide Fragments Platelet Membrane Glycoproteins Receptors, Cell Surface Fibrinogen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lantz M S
Switalski L M
Kornman K S
Höök M
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30 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.163.2.623-628.1985
Published
1985-08-00
Pages
623-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC219167
Subset
IM
Grants
NIAID NIH HHS · AI 20624 · United States
NIDCR NIH HHS · DE 07256 · United States
External Links
PubMed source DOI: 10.1128/jb.163.2.623-628.1985 PMC Full Text View journal details
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