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PMID: 2991586 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Intracellular location of rabbit poxvirus nucleic acid within infected cells as determined by in situ hybridization.

Journal of virology ·Vol. 55 ·No. 3 ·1985-09-00 ·Pages 634-43

Minnigan H, Moyer RW

Abstract

The intracellular location of rabbit poxvirus DNA within cells during the course of infection has been determined by the hybridization in situ of labeled viral DNA probes to uninfected and infected cells under various conditions. Extensive control experiments were performed to demonstrate that DNA could be detected selectively and accurately within the cell. Our results suggest that rabbit poxvirus DNA is located only within the cytoplasm during the reproductive cycle, and we found no evidence that viral DNA enters the cell nucleus. The pattern of hybridization of viral DNA at early times (1 and 2 h postinfection) and in the presence of inhibitors of viral DNA synthesis suggests that there may be an association between the input viral DNA and some structural component of the host cell. A number of observations support the hypothesis that the host cell nucleus is required for a productive poxvirus infection. Our results are discussed in terms of the possible role of the nucleus in the replication of poxviruses.

MeSH Terms
Animals Autoradiography Cell Nucleus/analysis Cells, Cultured Cycloheximide/pharmacology Cytoplasm/analysis DNA Replication DNA, Viral/analysis Humans Hydroxyurea/pharmacology Mice Nucleic Acid Hybridization Poxviridae/genetics Poxviridae Infections/microbiology RNA, Viral/analysis Vaccinia virus/genetics
Chemicals
DNA, Viral RNA, Viral Cycloheximide Hydroxyurea
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Minnigan H
Moyer R W
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22 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.55.3.634-643.1985
Published
1985-09-00
Pages
634-43
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255027
Subset
IM
Grants
NIAID NIH HHS · AI-15722 · United States
NIGMS NIH HHS · GM-07319 · United States
External Links
PubMed source DOI: 10.1128/JVI.55.3.634-643.1985 PMC Full Text View journal details
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