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PMID: 2991927 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Accurate transcription of cloned Neurospora RNA polymerase II-dependent genes in vitro by homologous soluble extracts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 16 ·1985-08-00 ·Pages 5450-4

Tyler BM, Giles NH

Abstract

We have developed soluble extracts from Neurospora crassa capable of accurately initiating the transcription of cloned Neurospora protein-encoding genes by RNA polymerase II in vitro. The genes encoding glutamate dehydrogenase (am) and histones H3 and H4 were transcribed by the extracts, and transcription was sensitive to alpha-amanitin at 1 mg/ml. The 5' heterogeneity of the in vitro initiation reactions was highly specific. Of the 17 transcription initiation sites within the inducible qa gene cluster, only one minor site was used in vitro, suggesting that, in general, transcription from qa gene promoters requires at least one different protein from those required for transcription of the am and histone genes.

MeSH Terms
Base Sequence Cloning, Molecular DNA Restriction Enzymes Genes Genes, Fungal Kinetics Neurospora/genetics Neurospora crassa/enzymology,genetics Plasmids RNA Polymerase II/metabolism RNA, Messenger/genetics Templates, Genetic Transcription, Genetic
Chemicals
RNA, Messenger RNA Polymerase II DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tyler B M
Giles N H
References (25)
25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.16.5450
Published
1985-08-00
Pages
5450-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390587
Subset
IM
Grants
NIGMS NIH HHS · GM28777 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.16.5450 PMC Full Text View journal details
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