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PMID: 2992451 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Simultaneous purification and characterization of glucokinase, fructokinase and glucose-6-phosphate dehydrogenase from Zymomonas mobilis.

The Biochemical journal ·Vol. 228 ·No. 3 ·1985-06-15 ·Pages 627-34

Scopes RK, Testolin V, Stoter A, Griffiths-Smith K, Algar EM

Abstract

The three enzymes glucokinase (EC 2.7.1.2), fructokinase (EC 2.7.1.4) and glucose-6-phosphate dehydrogenase (EC 1.1.1.49) were isolated in high yield from extracts of Zymomonas mobilis. The principal steps in the isolation procedures involved the use of selected dye-ligand adsorbent columns, with affinity elution of two of the three enzymes. Glucokinase and fructokinase are dimeric proteins (2 X 33000 Da and 2 X 28000 Da respectively) and glucose-6-phosphate dehydrogenase is a tetramer (4 X 52000 Da). Some similarities in the structural and kinetic parameters of the two kinases were noted, but they have absolute specificity for their substrates. Fructokinase is strongly inhibited by glucose; otherwise non-substrate sugars had little effect on any of the three enzymes.

MeSH Terms

Adsorption Bacteria/enzymology Carbohydrate Metabolism Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Fructokinases/isolation & purification,metabolism Glucokinase/isolation & purification,metabolism Glucosephosphate Dehydrogenase/isolation & purification,metabolism Kinetics Macromolecular Substances Phosphotransferases/metabolism Substrate Specificity

Chemicals

Macromolecular Substances Glucosephosphate Dehydrogenase Phosphotransferases Fructokinases Glucokinase fructokinase

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Scopes R K

Testolin V

Stoter A

Griffiths-Smith K

Algar E M

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24 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2280627

Published

1985-06-15

Pages

627-34

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1145031

Subset

External Links

PubMed source DOI: 10.1042/bj2280627 PMC Full Text View journal details

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Simultaneous purification and characterization of glucokinase, fructokinase and glucose-6-phosphate dehydrogenase from Zymomonas mobilis.

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