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PMID: 2992937 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Erythroid-specific expression of human beta-globin genes in transgenic mice.

The EMBO journal ·Vol. 4 ·No. 7 ·1985-07-00 ·Pages 1715-23

Townes TM, Lingrel JB, Chen HY, Brinster RL, Palmiter RD

Abstract

Transgenic mice carrying human beta-globin genes were produced by microinjecting linear DNA molecules containing cloned beta-globin genes with up to 4300 bp of 5'-flanking sequence and 1700 bp of 3'-flanking sequence. Most (15 of 20) of these transgenic mice expressed the human beta-globin genes in blood cells and the level of expression in some mice was comparable with that obtained from endogenous beta-globin genes. Human beta-globin gene expression appeared to be restricted to cells of the erythroid lineage and was first detected between 11 and 14 days of development, in parallel with mouse beta-globin. Constructs with as little as 48 bp of 5'-flanking sequence also appeared to be expressed appropriately. The mRNA transcripts had correct 5' ends and directed human beta-globin synthesis in reticulocyte lysates. Human beta-globin protein was detectable in mature erythrocytes from progeny of one of these mice. The frequency and extent of expression was severely depressed when the procaryotic vector DNA was not removed prior to microinjection.

MeSH Terms
Animals Base Sequence DNA/genetics DNA Restriction Enzymes Genes Globins/genetics Humans Mice Mice, Inbred Strains Microinjections Nucleic Acid Hybridization Protein Biosynthesis RNA, Messenger/genetics Reticulocytes/metabolism Transcription, Genetic
Chemicals
RNA, Messenger Globins DNA DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Townes T M
Lingrel J B
Chen H Y
Brinster R L
Palmiter R D
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36 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-07-00
Pages
1715-23
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554408
Subset
IM
Grants
NIGMS NIH HHS · GM-10999 · United States
NICHD NIH HHS · HD-09172 · United States
NICHD NIH HHS · HD-17321 · United States
External Links
PubMed source PMC Full Text View journal details
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