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PMID: 2992943 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Common features of polyomavirus mutants selected on PCC4 embryonal carcinoma cells.

The EMBO journal ·Vol. 4 ·No. 7 ·1985-07-00 ·Pages 1799-803

Melin F, Pinon H, Reiss C, Kress C, Montreau N, Blangy D

Abstract

The genomic rearrangements of six polyomavirus mutants selected on PCC4 embryonal carcinoma cells have been compared and their common characteristics pointed out. All mutants show a duplication which includes at least the adenovirus type 5 (Ad5) E1A-like enhancer core sequence plus a deletion of variable size and location. The presence of the second enhancer core sequence, the SV40-like enhancer, is not required for expression of the PyEC PCC4 phenotype. Two of these mutants are also able to express polyomavirus T antigen on F9 and LT1 cells. Multiadaptation seems to require the duplication of the Ad5 E1A-like core sequence, the maintenance of the SV40-like core sequence and a local change in DNA stability.

MeSH Terms
Animals Base Sequence Cell Line Cloning, Molecular DNA Restriction Enzymes Mice Mutation Polyomavirus/genetics Teratoma/microbiology
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Melin F
Pinon H
Reiss C
Kress C
Montreau N
Blangy D
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33 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-07-00
Pages
1799-803
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554420
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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