Home LiteratureArticle Details
PMID: 2993319 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Reciprocal transmembranous receptor-cytoskeleton interactions in concanavalin A-activated platelets.

The Journal of cell biology ·Vol. 101 ·No. 3 ·1985-09-00 ·Pages 993-1000

Wheeler ME, Gerrard JM, Carroll RC

Abstract

Concanavalin A (Con A) has been used to activate platelets, inducing a specific interaction between the glycoprotein IIb-IIIa complex and the cytoskeleton of the activated platelet. In agreement with this, we have shown that Con A activates human platelets, initiating phosphorylation, secretion, and cytoskeletal formation. Con A and cytochalasin B were used to demonstrate a reciprocal interaction of the glycoprotein complex with the platelet cytoskeleton. Additionally, we have shown that a similar reciprocity is provided by the multivalent fibrin-fibrinogen platelet interaction found in the thrombin-induced clot. Con A differs from other activators in precipitating an apparent cytoskeletal core despite a complete inhibition of platelet activation by prostaglandin E1. We suggest, from this result, that Con A may be cross-linking a membrane-associated cytoskeletal complex present in the unactivated platelet.

MeSH Terms
Actins/metabolism Alprostadil Blood Platelets/drug effects,metabolism,ultrastructure Carrier Proteins/metabolism Concanavalin A/pharmacology Cytoskeleton/metabolism Fibrinogen/metabolism Gelsolin Glycoproteins/metabolism Humans Membrane Proteins/metabolism Microfilament Proteins Myosins/metabolism Phosphorylation Platelet Aggregation Platelet Membrane Glycoproteins Prostaglandins E/pharmacology Receptors, Cell Surface/metabolism
Chemicals
Actins Carrier Proteins Gelsolin Glycoproteins Membrane Proteins Microfilament Proteins Platelet Membrane Glycoproteins Prostaglandins E Receptors, Cell Surface brevin Concanavalin A Fibrinogen Myosins Alprostadil
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wheeler M E
Gerrard J M
Carroll R C
References (45)
45 references, click to expand
  1. Mechanism of concanavalin A-induced anchorage of the major cell surface glycoproteins to the submembrane cytoskeleton in 13762 ascites mammary adenocarcinoma cells.
    J Cell Biol. 1984 Jan;98(1):179-87 PMID: 6538571
  2. Ca2+-activated, phospholipid-dependent protein kinase catalyzes the phosphorylation of actin-binding proteins.
    Biochem Biophys Res Commun. 1984 Feb 14;118(3):736-42 PMID: 6231024
  3. Association of fibrin with the platelet cytoskeleton.
    J Biol Chem. 1984 Apr 25;259(8):5247-54 PMID: 6325426
  4. Activation of phospholipase C in thrombin-stimulated platelets does not depend on cytoplasmic free calcium concentration.
    FEBS Lett. 1984 May 7;170(1):43-8 PMID: 6327376
  5. Actin filament content and organization in unstimulated platelets.
    J Cell Biol. 1984 Jun;98(6):1985-91 PMID: 6233292
  6. Cytoplasmic Ca2+ in platelets is controlled by cyclic AMP: antagonism between stimulators and inhibitors of adenylate cyclase.
    Biochem Biophys Res Commun. 1984 Apr 30;120(2):579-85 PMID: 6329175
  7. Platelet activation induces the formation of a stable gelsolin-actin complex from monomeric gelsolin.
    J Biol Chem. 1984 Jun 25;259(12):7473-9 PMID: 6330059
  8. Evidence for the presence of tropomyosin in the cytoskeletons of ADP- and thrombin-stimulated blood platelets.
    FEBS Lett. 1984 Jul 23;173(1):164-8 PMID: 6540206
  9. Retention of the glycoprotein IIb-IIIa complex in the isolated platelet cytoskeleton. Effects of separable assembly of platelet pseudopodal and contractile cytoskeletons.
    J Clin Invest. 1984 Sep;74(3):1080-9 PMID: 6088580
  10. myo-Inositol 1,4,5-trisphosphate stimulates protein phosphorylation in saponin-permeabilized human platelets.
    Proc Natl Acad Sci U S A. 1984 Dec;81(23):7431-5 PMID: 6438636
  11. On the association of glycoprotein Ib and actin-binding protein in human platelets.
    J Cell Biol. 1985 Jan;100(1):317-21 PMID: 3155520
  12. Isolation of a subpopulation of glycoprotein IIb-III from platelet membranes that is bound to membrane actin.
    J Cell Biol. 1985 Feb;100(2):652-7 PMID: 3155751
  13. Platelet cytoskeleton--membrane interactions.
    Biochem Biophys Res Commun. 1985 Jan 16;126(1):566-70 PMID: 4038601
  14. Analysis of bacteriophage T7 early RNAs and proteins on slab gels.
    J Mol Biol. 1973 Sep 15;79(2):237-48 PMID: 4760132
  15. Concanavalin-A-induced transmembrane linkage of concanavalin A surface receptors to intracellular myosin-containing filaments.
    Proc Natl Acad Sci U S A. 1976 Dec;73(12):4575-9 PMID: 1070007
  16. Actin co-caps with concanavalin A receptors.
    Nature. 1977 Oct 20;269(5630):695-7 PMID: 593328
  17. Platelet actin: sub-cellular distribution and association with profilin.
    FEBS Lett. 1978 Jun 1;90(1):84-8 PMID: 658445
  18. The role of lipids in platelet function: with particular reference to the arachidonic acid pathway.
    J Lipid Res. 1978 Sep;19(7):793-826 PMID: 101634
  19. Ultrastructural changes of lectin-stimulated platelets analyzed by thin sections and freeze-fracturing.
    J Ultrastruct Res. 1978 Dec;65(3):234-45 PMID: 731789
  20. Characterization of a platelet protein phosphorylated during the thrombin-induced release reaction.
    Biochemistry. 1979 Feb 6;18(3):544-52 PMID: 420799
  21. Lysophosphatidic acids. Influence on platelet aggregation and intracellular calcium flux.
    Am J Pathol. 1979 Aug;96(2):423-38 PMID: 112871
  22. A study of protein phosphorylation in shape change and Ca++-dependent serotonin release by blood platelets.
    Cell. 1979 Dec;18(4):1015-23 PMID: 519763
  23. Identification of human platelet glycoproteins in SDS-polyacrylamide gels using 125I labelled lectins.
    Thromb Res. 1979;16(5-6):825-31 PMID: 524323
  24. Subcellular distribution of the different platelet proteins phosphorylated on exposure of intact platelets to ionophore A23187 or to prostaglandin E1. Possible role of a membrane phosphopolypeptide in the regulation of calcium-ion transport.
    Biochem J. 1979 Dec 15;184(3):651-61 PMID: 120200
  25. Identification of membrane proteins mediating the interaction of human platelets.
    J Cell Biol. 1980 Jul;86(1):77-86 PMID: 6893455
  26. Actin-binding protein promotes the bipolar and perpendicular branching of actin filaments.
    J Cell Biol. 1980 Dec;87(3 Pt 1):841-8 PMID: 6893990
  27. Characterization of platelet extracts before and after stimulation with respect to the possible role of profilactin as microfilament precursor.
    Cell. 1981 Jan;23(1):145-53 PMID: 6783315
  28. Platelet activation and microfilament bundling.
    J Cell Biol. 1981 Apr;89(1):146-51 PMID: 7194875
  29. Myosin phosphorylation in intact platelets.
    J Biol Chem. 1981 Jul 25;256(14):7510-4 PMID: 7251606
  30. Transmembrane linkage between surface glycoproteins and components of the cytoplasm in neutrophil leukocytes.
    J Cell Biol. 1981 Sep;90(3):743-54 PMID: 7197281
  31. Phosphorylation of platelet actin-binding protein during platelet activation.
    Blood. 1982 Mar;59(3):466-71 PMID: 7199358
  32. Concanavalin A induces interactions between surface glycoproteins and the platelet cytoskeleton.
    J Cell Biol. 1982 Feb;92(2):565-73 PMID: 6460776
  33. Role of phosphorylation in mediating the association of myosin with the cytoskeletal structures of human platelets.
    J Biol Chem. 1982 Apr 25;257(8):4120-6 PMID: 7040379
  34. Cytochalasin D and platelet gelsolin accelerate actin polymer formation. A model for regulation of the extent of actin polymer formation in vivo.
    Biochemistry. 1982 Jun 22;21(13):3207-14 PMID: 6285961
  35. Are lysophosphatidic acids or phosphatidic acids involved in stimulus activation coupling in platelets?
    Blood. 1982 Sep;60(3):642-9 PMID: 6921047
  36. Evidence that changes in platelet cyclic AMP levels regulate the fibrinogen receptor on human platelets.
    J Biol Chem. 1982 Dec 25;257(24):14606-9 PMID: 6294072
  37. Reversal of thrombin-induced myosin phosphorylation and the assembly of cytoskeletal structures in platelets by the adenylate cyclase stimulants prostaglandin D2 and forskolin.
    J Biol Chem. 1983 Jan 25;258(2):1260-7 PMID: 6571835
  38. A role of calcium-activated phospholipid-dependent protein kinase in human platelet activation. Comparison of thrombin and collagen actions.
    J Biol Chem. 1983 Feb 10;258(3):2010-3 PMID: 6218169
  39. The thromboxane antagonist, 13-azaprostanoic acid, inhibits arachidonic acid-induced Ca2+ release from isolated platelet membrane vesicles.
    Biochim Biophys Acta. 1983 Mar 22;751(1):66-73 PMID: 6830832
  40. The microfilament network of the platelet.
    Prog Hemost Thromb. 1982;6:63-84 PMID: 6300966
  41. Isolation and some structural and functional properties of macrophage tropomyosin.
    Biochemistry. 1983 Mar 1;22(5):1187-93 PMID: 6838847
  42. Elevation of cytoplasmic free calcium concentration by stable thromboxane A2 analogue in human platelets.
    Biochem Biophys Res Commun. 1983 Dec 28;117(3):663-9 PMID: 6230078
  43. alpha-Actinin and vinculin in normal and thrombasthenic platelets.
    Blood. 1984 Mar;63(3):606-14 PMID: 6230120
  44. Role of actin binding protein phosphorylation in platelet cytoskeleton assembly.
    Biochem Biophys Res Commun. 1984 Jan 30;118(2):508-13 PMID: 6367740
  45. Recycling of platelet phosphorylation and cytoskeletal assembly.
    J Cell Biol. 1984 Jan;98(1):8-15 PMID: 6423649
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.101.3.993
Published
1985-09-00
Pages
993-1000
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113701
Subset
IM
Grants
NHLBI NIH HHS · HL07430 · United States
NHLBI NIH HHS · HL34445-01 · United States
External Links
PubMed source DOI: 10.1083/jcb.101.3.993 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2