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PMID: 2993817 Published · ppublish English Journal Article

ISL1: a new transposable element in Lactobacillus casei.

Molecular & general genetics : MGG ·Vol. 200 ·No. 2 ·1985-00-00 ·Pages 193-8

Shimizu-Kadota M, Kiwaki M, Hirokawa H, Tsuchida N

Abstract

The genome structures of a temperate Lactobacillus phage, phi FSW, and its virulent mutants, phi FSVs, were examined by restriction, heteroduplex and nucleotide-sequence analyses. The results showed that two out of three phi FSVs had the same 1.3 kbp insertion (designated as ISL1) at different positions in the phi FSW sequence. ISL1 was 1,256 bp long and contained at least two long open reading frames of 279 and 822 bases on one strand. Inverted repeats were found at the termini of the ISL1 which was bracketed by 3 bp direct repeats of the phi FSW sequence. From this evidence, we concluded that ISL1 was a transposable element in Lactobacillus casei.

MeSH Terms
Bacteriophages/genetics Base Composition Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA Transposable Elements Genes, Bacterial Genes, Viral Lactobacillus casei/genetics Microscopy, Electron
Chemicals
DNA Transposable Elements DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shimizu-Kadota M
Kiwaki M
Hirokawa H
Tsuchida N
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28 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00425423
Published
1985-00-00
Pages
193-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X02734
External Links
PubMed source DOI: 10.1007/BF00425423
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