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PMID: 2993870 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Host species specificity of polyomavirus DNA replication is not altered by simian virus 40 72-base-pair repeats.

Molecular and cellular biology ·Vol. 5 ·No. 6 ·1985-06-00 ·Pages 1534-7

Campbell BA, Villarreal LP

Abstract

The simian virus 40 72-base-pair repeats substituted for the polyomavirus enhancer, allowing replication and transcription in mouse 3T6 but not monkey CV-1 cells. A polyomavirus genome containing the entire simian virus 40 control region replicated at low levels in 3T6 and CV-1 cells; however, transcripts were detected only in 3T6 cells. Our results suggest that the simian virus 40 72-base-pair repeats are unable to alter the host species specificity of the complete polyomavirus genome.

MeSH Terms
Animals Cell Line Chlorocebus aethiops DNA Replication Enhancer Elements, Genetic Genes, Regulator Genes, Viral Mice Polyomavirus/genetics Repetitive Sequences, Nucleic Acid Simian virus 40/genetics Species Specificity Transcription, Genetic Virus Replication
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Campbell B A
Villarreal L P
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22 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.6.1534-1537.1985
Published
1985-06-00
Pages
1534-7
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366888
Subset
IM
Grants
PHS HHS · 2532413 · United States
NIAID NIH HHS · 5T32AI07209 · United States
NCI NIH HHS · CA2532422 · United States
External Links
PubMed source DOI: 10.1128/mcb.5.6.1534-1537.1985 PMC Full Text View journal details
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