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PMID: 2994009 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of the Bacillus subtilis xylose isomerase gene: extensive homology between the Bacillus and Escherichia coli enzyme.

Nucleic acids research ·Vol. 13 ·No. 15 ·1985-08-12 ·Pages 5717-22

Wilhelm M, Hollenberg CP

Abstract

The xylose isomerase gene from Bacillus subtilis was cloned from a genomic BamH1 library by complementation of an isomerase defective Escherichia coli strain as previously described. The ATG initiation codon is preceded by a Shine-Dalgarno sequence and two hexamers being characteristic for the promoter region of Bacillus genes. The structural gene consists of 1320 base pairs, thus coding for a polypeptide chain of 440 amino acids with a molecular weight of 49 680. The polypeptide primary structure shows over 50% homology to that of the E. coli xylose isomerase.

MeSH Terms

Aldose-Ketose Isomerases Bacillus subtilis/enzymology,genetics Base Sequence Carbohydrate Epimerases/genetics Codon Escherichia coli/enzymology,genetics Operon Phosphotransferases/genetics Phosphotransferases (Alcohol Group Acceptor)

Chemicals

Codon Phosphotransferases Phosphotransferases (Alcohol Group Acceptor) xylulokinase Carbohydrate Epimerases Aldose-Ketose Isomerases xylose isomerase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Wilhelm M

Hollenberg C P

References (11)

11 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/13.15.5717

Published

1985-08-12

Pages

5717-22

Language

English

Region

England

NLM ID

0411011

PMCID

PMC321901

Subset

Databases

GENBANK

X01109, X02795

External Links

PubMed source DOI: 10.1093/nar/13.15.5717 PMC Full Text View journal details

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Nucleotide sequence of the Bacillus subtilis xylose isomerase gene: extensive homology between the Bacillus and Escherichia coli enzyme.

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