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PMID: 2994051 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Glucocorticoid regulation of mouse mammary tumor virus sequences in transgenic mice.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 17 ·1985-09-00 ·Pages 5880-4

Ross SR, Solter D

Abstract

We have introduced a chimeric plasmid, pLTR2TK, containing the mouse mammary tumor virus (MTV) long terminal repeat (LTR) linked to the herpes simplex virus type 1 thymidine kinase gene into the mouse germ line by microinjection. In one mouse line, the thymidine kinase gene is appropriately expressed in the lactating mammary glands of heterozygous females; expression also occurs in the ovaries of these mice. In heterozygous males of this line, and in a male derived from another microinjection, transcription of these new germ line genes occurs in the testes and is specifically stimulated by glucocorticoid hormones. Thus, the MTV LTR may contain elements that allow its expression both in lactating mammary glands and in testicular tissue.

MeSH Terms
Animals DNA, Recombinant DNA, Viral/genetics Female Gene Expression Regulation Genes, Viral Genetic Engineering Glucocorticoids/physiology Lactation Male Mammary Tumor Virus, Mouse/genetics Mice Ovary/physiology Plasmids Pregnancy Promoter Regions, Genetic Repetitive Sequences, Nucleic Acid Testis/physiology Thymidine Kinase/genetics Transcription, Genetic
Chemicals
DNA, Recombinant DNA, Viral Glucocorticoids Thymidine Kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ross S R
Solter D
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37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.17.5880
Published
1985-09-00
Pages
5880-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390657
Subset
IM
Grants
PHS HHS · 48314 · United States
NICHD NIH HHS · HD 12487 · United States
NICHD NIH HHS · HD 19444 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.17.5880 PMC Full Text View journal details
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