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PMID: 2994056 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Detection of a raf-related and two other transforming DNA sequences in human tumors maintained in nude mice.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 17 ·1985-09-00 ·Pages 5954-8

Fukui M, Yamamoto T, Kawai S, Maruo K, Toyoshima K

Abstract

High molecular weight DNAs prepared from a variety of human tumors maintained in nude mice were assayed for their ability to transform NIH 3T3 cells. DNAs from 4 of 21 tumors tested induced transformed foci in cultures of NIH 3T3 cells. They were from a Ewing sarcoma line, a glioblastoma line, a leiomyosarcoma line, and a lung carcinoma line. Hybridization analyses of the NIH 3T3 transformant DNAs with a human repetitive sequence as probe revealed that four distinct transforming DNA sequences were transferred to NIH 3T3 cells from the four tumor lines. The transforming DNA in a lung carcinoma line was a human homologue of the oncogene of Kirsten murine sarcoma virus (Ki-ras). On the other hand, the three other transforming DNAs showed no similarity to any known human transforming gene detected by the NIH 3T3 transformation assay. Further analyses with a series of cloned oncogenes as probes revealed that the transforming DNA in a glioblastoma line was a human homologue of the oncogene of 3611-murine sarcoma virus (raf). However, the two transforming DNAs in a Ewing sarcoma line and a leiomyosarcoma line had no sequence homology to any of the cloned oncogenes.

MeSH Terms
Animals Cell Transformation, Neoplastic DNA Restriction Enzymes DNA, Neoplasm/genetics Humans Mice Mice, Nude Neoplasms, Experimental/genetics Oncogenes Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Neoplasm DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Fukui M
Yamamoto T
Kawai S
Maruo K
Toyoshima K
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42 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.17.5954
Published
1985-09-00
Pages
5954-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390672
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.17.5954 PMC Full Text View journal details
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