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PMID: 2995247 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

HLA-DR genotyping by restriction fragment length polymorphism analyses.

Immunogenetics ·Vol. 22 ·No. 3 ·1985-00-00 ·Pages 231-9

Hui K, Festenstein H, de Klein A, Grosveld G, Grosveld F

Abstract

We have established unique restriction fragment length polymorphism (RFLP) patterns characteristic of homozygous typing cells (HTCs) for HLA-DR-1 through HLA-DR-8 haplotypes. These RFLP patterns were found to segregate in family members and correlate 100% with HLA-DR antibody phenotyping. The RFLP patterns were used to type chronic myelocytic leukemic cells which have a Philadelphia translocation from 23 randomly selected Caucasoid patients. The results show an alternative method for the determination of the HLA-DR types without using live cells and to study disease association with the HLA-DR region.

MeSH Terms
Cell Line DNA Restriction Enzymes Genotype HLA-DR Antigens Histocompatibility Antigens Class II/genetics Humans Nucleic Acid Hybridization Pedigree Philadelphia Chromosome Polymorphism, Genetic
Chemicals
HLA-DR Antigens Histocompatibility Antigens Class II DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hui K
Festenstein H
de Klein A
Grosveld G
Grosveld F
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22 references, click to expand
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Article Info
Journal
Immunogenetics
Abbr.
Immunogenetics
ISSN
0093-7711
DOI
10.1007/BF00404482
Published
1985-00-00
Pages
231-9
Language
English
Region
United States
NLM ID
0420404
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00404482 View journal details
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