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PMID: 2997233 Published · ppublish English Journal Article

Large-scale purification of presynaptic plasma membranes from Torpedo marmorata electric organ.

The Journal of cell biology ·Vol. 101 ·No. 5 Pt 1 ·1985-11-00 ·Pages 1757-62

Morel N, Marsal J, Manaranche R, Lazereg S, Mazie JC, Israel M

Abstract

The presynaptic plasma membrane (PSPM) of cholinergic nerve terminals was purified from Torpedo electric organ using a large-scale procedure. Up to 500 g of frozen electric organ were fractioned in a single run, leading to the isolation of greater than 100 mg of PSPM proteins. The purity of the fraction is similar to that of the synaptosomal plasma membrane obtained after subfractionation of Torpedo synaptosomes as judged by its membrane-bound acetylcholinesterase activity, the number of Glycera convoluta neurotoxin binding sites, and the binding of two monoclonal antibodies directed against PSPM. The specificity of these antibodies for the PSPM is demonstrated by immunofluorescence microscopy.

MeSH Terms
Acetylcholinesterase/analysis Animals Antibodies, Monoclonal Cell Fractionation/methods Cell Membrane/metabolism,ultrastructure Electric Organ/ultrastructure Microscopy, Electron Neuromuscular Junction/ultrastructure Neurotoxins/analysis Receptors, Cholinergic/analysis Receptors, Nicotinic/analysis Synapses/ultrastructure Torpedo
Chemicals
Antibodies, Monoclonal Neurotoxins Receptors, Cholinergic Receptors, Nicotinic Acetylcholinesterase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Morel N
Marsal J
Manaranche R
Lazereg S
Mazie J C
Israel M
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31 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.101.5.1757
Published
1985-11-00
Pages
1757-62
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113949
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.101.5.1757 PMC Full Text View journal details
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