Target-cell membrane alterations induced by lymphotoxin. Ultrastructural observations.
Upon in vitro stimulation by antigens or mitogens, lymphocytes release a series of lymphokines. One such lymphocyte mediator is lymphotoxin, which appears to be responsible for in vitro lymphocyte-mediated cytolysis. Employing immuno-electron microscopy with ferritin- or peroxidase-labeled antibody, we observed patchy localization of the mediator on the plasma membranes of target L cells exposed to lymphotoxin, often in areas overlying a microfilament web. When studying lymphotoxin-affected cells by electron microscopy of freeze-fracture replicas, we observed aggrlasma membrane, with intervening areas which were frequently particle free. Sometimes the affected cells revealed plasma-membrane lesions suggesting intramembranous "blisters."
19 references, click to expand
-
Chemical characterization and surface orientation of the major glycoprotein of the human erythrocyte membrane.Proc Natl Acad Sci U S A. 1972 Jun;69(6):1445-9 PMID: 4504356
-
The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.J Histochem Cytochem. 1966 Apr;14(4):291-302 PMID: 5962951
-
Early biochemical alterations induced by lymphotoxin in target cells.J Immunol. 1973 Oct;111(4):1128-35 PMID: 4728679
-
The fluid mosaic model of the structure of cell membranes.Science. 1972 Feb 18;175(4023):720-31 PMID: 4333397
-
Membrane structure of Entamoeba histolytica: fine structure of freeze-fractured membranes.J Ultrastruct Res. 1976 Jan;54(1):148-58 PMID: 175169
-
Comparison and yield of antigen- or mitogen-induced human lymphotoxins.J Immunol. 1974 Apr;112(4):1347-53 PMID: 4273626
-
Lymphotoxin. A review and analysis.Am J Pathol. 1976 Sep;84(3):580-96 PMID: 786035
-
Cellular receptors for lymphotoxin: correlation of binding and cytotoxicity in sensitive and resistant target cells.J Immunol. 1976 Jan;116(1):184-7 PMID: 1082000
-
Alteratons in RNA synthesis in lymphotoxin-treated target cells.Am J Pathol. 1975 Aug;80(2):249-60 PMID: 1080634
-
Ultrastructure of membrane lesions in immune lysis, osmotic lysis and drug-induced lysis.Fed Proc. 1974 Oct;33(10):2116-24 PMID: 4607618
-
Role of soluble cytotoxins in cell-mediated immunity.Transplant Proc. 1973 Mar;5(1):137-40 PMID: 4572090
-
Biological membranes as bilayer couples. A molecular mechanism of drug-erythrocyte interactions.Proc Natl Acad Sci U S A. 1974 Nov;71(11):4457-61 PMID: 4530994
-
Fracture faces of osmotically disrupted zonulae occludentes.J Cell Biol. 1974 Aug;62(2):344-50 PMID: 4473455
-
Membrane splitting in freeze-ethching. Covalently bound ferritin as a membrane marker.J Cell Biol. 1970 Jun;45(3):598-605 PMID: 4918216
-
Membrane-microtubule interactions: concanavalin A capping induced redistribution of cytoplasmic microtubules and colchicine binding proteins.Proc Natl Acad Sci U S A. 1975 Dec;72(12):4976-80 PMID: 1061084
-
Plasma membrane changes in freeze-fractured rat kidney cortex following renal ischemia.Lab Invest. 1976 Jul;35(1):63-70 PMID: 940322
-
Lymphocyte in vitro cytotoxicity: mechanism of lymphotoxin-induced target cell destruction.J Immunol. 1969 Apr;102(4):911-8 PMID: 5813967
-
Enzyme-labeled antibodies for the light and electron microscopic localization of tissue antigens.J Cell Biol. 1967 May;33(2):307-18 PMID: 6046571
-
Cytolysis induced by human lymphotoxin.Am J Pathol. 1972 Oct;69(1):103-18 PMID: 4562794
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