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PMID: 2999712 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genomic cloning and characterization of a ricin gene from Ricinus communis.

Nucleic acids research ·Vol. 13 ·No. 22 ·1985-11-25 ·Pages 8019-33

Halling KC, Halling AC, Murray EE, Ladin BF, Houston LL, Weaver RF

Abstract

A genomic clone that specifies a single polypeptide precursor for ricin, a toxic lectin of Ricinus communis (castor bean), was isolated, sequenced and Sl mapped. The gene encodes a 64 kDa precursor which contains, in the following order: a 24 or 35 amino acid signal peptide, the A chain, a 12 amino acid linker peptide, and the B chain. The 5'-end of the ricin mRNA maps approximately 35 bases upstream from the first methionine codon. Two putative TATA boxes and a possible CAAT box lie in the 5'-flanking region. Two possible polyadenylation signals were found in the 3' flanking region. No introns were found, which is typical of other lectin genes that have been sequenced. Southern blot analysis suggests that the castor bean genome contains approximately six ricin-like genes.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA/genetics DNA Restriction Enzymes Protein Precursors/genetics Protein Sorting Signals/genetics Ricin/genetics Transcription, Genetic
Chemicals
Protein Precursors Protein Sorting Signals DNA Ricin DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Halling K C
Halling A C
Murray E E
Ladin B F
Houston L L
Weaver R F
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33 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.22.8019
Published
1985-11-25
Pages
8019-33
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322107
Subset
IM
Databases
GENBANK
X03179
External Links
PubMed source DOI: 10.1093/nar/13.22.8019 PMC Full Text View journal details
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