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PMID: 2999713 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Functional activity and chromatin configuration of SV40 enhancer injected in Xenopus laevis oocytes.

Nucleic acids research ·Vol. 13 ·No. 22 ·1985-11-25 ·Pages 8065-81

Spinelli G, Ciliberto G

Abstract

The SV40 enhancer microinjected into the nuclei of X. laevis oocytes is able to activate transcription about 100-fold when cloned upstream of the SV40 early promoter region or about 10-fold regardless of its orientation when located at a long distance from a test gene (bacterial chloramphenicol acetyl transferase). This effect is qualitatively and quantitatively similar to that observed when analogous constructions were transfected into mammalian cells. Making use of a direct labelling technique for the analysis of the chromatin structure we could show that the SV40 enhancer region in the microinjected plasmids is particularly accessible to cleavage with MNase or DNAse I. Single sites in the 72 bp repeats have been mapped at the nucleotide level.

MeSH Terms
Animals Base Sequence Chromatin/ultrastructure DNA, Viral/metabolism Deoxyribonuclease I/metabolism Enhancer Elements, Genetic Female Genes, Regulator Micrococcal Nuclease/metabolism Microinjections Nucleosomes/ultrastructure Oocytes/metabolism Plasmids Simian virus 40/genetics Species Specificity Transcription, Genetic Xenopus laevis
Chemicals
Chromatin DNA, Viral Nucleosomes Deoxyribonuclease I Micrococcal Nuclease
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Spinelli G
Ciliberto G
References (38)
38 references, click to expand
  1. Enhancer elements.
    Cell. 1983 Jun;33(2):313-4 PMID: 6305503
  2. Transcriptional 'enhancers' from SV40 and polyoma virus show a cell type preference.
    Nucleic Acids Res. 1982 Dec 20;10(24):7965-76 PMID: 6298703
  3. A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes.
    Cell. 1983 Jul;33(3):729-40 PMID: 6409418
  4. Immunoglobulin gene transcription is activated by downstream sequence elements.
    Cell. 1983 Jul;33(3):741-8 PMID: 6409419
  5. Simian virus 40 early- and late-region promoter functions are enhanced by the 72-base-pair repeat inserted at distant locations and inverted orientations.
    Mol Cell Biol. 1983 Jun;3(6):991-9 PMID: 6308429
  6. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  7. The use of Xenopus oocytes for the expression of cloned genes.
    Methods Enzymol. 1983;101:370-86 PMID: 6193395
  8. Analysis of the chromatin assembled in germinal vesicles of Xenopus oocytes.
    J Mol Biol. 1983 Nov 5;170(3):699-722 PMID: 6415291
  9. Cell-specific expression controlled by the 5'-flanking region of insulin and chymotrypsin genes.
    Nature. 1983 Dec 8-14;306(5943):557-61 PMID: 6358900
  10. Chromatin structure of simian virus 40-pBR322 recombinant plasmids in COS-1 cells.
    Mol Cell Biol. 1983 Dec;3(12):2203-10 PMID: 6318089
  11. Specific interaction between enhancer-containing molecules and cellular components.
    Cell. 1984 Feb;36(2):403-11 PMID: 6319021
  12. Stimulation of in vitro transcription from heterologous promoters by the simian virus 40 enhancer.
    Proc Natl Acad Sci U S A. 1984 Jan;81(2):308-12 PMID: 6320179
  13. Magic enhancers?
    DNA. 1984;3(1):1-5 PMID: 6321119
  14. Induction of altered chromatin structures by simian virus 40 enhancer and promoter elements.
    Nature. 1984 Feb 23-29;307(5953):708-14 PMID: 6321997
  15. Assembly of transfected DNA into chromatin: structural changes in the origin-promoter-enhancer region upon replication.
    EMBO J. 1984 Jun;3(6):1243-53 PMID: 6086311
  16. The MLV and SV40 enhancers have a similar pattern of transcriptional activation.
    Nucleic Acids Res. 1984 Dec 11;12(23):8801-18 PMID: 6096807
  17. A trans-acting factor is responsible for the simian virus 40 enhancer activity in vitro.
    Nature. 1985 Feb 7-13;313(6002):458-63 PMID: 2982105
  18. Simian virus 40 enhancer increases number of RNA polymerase II molecules on linked DNA.
    Nature. 1985 May 2-8;315(6014):73-5 PMID: 2986013
  19. Simian virus 40 enhancer increases RNA polymerase density within the linked gene.
    Nature. 1985 May 2-8;315(6014):75-7 PMID: 2986014
  20. A viral enhancer element specifically active in human haematopoietic cells.
    Nature. 1985 Jun 13-19;315(6020):597-600 PMID: 2989696
  21. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  22. Selective DNA conservation and chromatin assembly after injection of SV40 DNA into Xenopus oocytes.
    Dev Biol. 1978 May;64(1):178-88 PMID: 207607
  23. Transcription of cloned Xenopus ribosomal genes visualised after injection into oocyte nuclei.
    Nature. 1978 Nov 16;276(5685):292-4 PMID: 568722
  24. Sites in simian virus 40 chromatin which are preferentially cleaved by endonucleases.
    Cell. 1978 Dec;15(4):1511-8 PMID: 215331
  25. A stretch of "late" SV40 viral DNA about 400 bp long which includes the origin of replication is specifically exposed in SV40 minichromosomes.
    Cell. 1979 Feb;16(2):453-66 PMID: 222461
  26. Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9.
    Nature. 1979 Dec 20-27;282(5741):864-9 PMID: 390403
  27. A nucleosome-free region in SV40 minichromosomes.
    Nature. 1980 May 22;285(5762):263-5 PMID: 6246449
  28. Absence of nucleosomes in a fraction of SV40 chromatin between the origin of replication and the region coding for the late leader RNA.
    Cell. 1980 May;20(1):65-73 PMID: 6248237
  29. Regulated replication of DNA microinjected into eggs of Xenopus laevis.
    Cell. 1980 Oct;21(3):761-71 PMID: 6254667
  30. Fine structure of the origin-proximal DNAase I-hypersensitive region in wild-type and EC mutant polyoma.
    Cell. 1981 Sep;25(3):651-8 PMID: 6269751
  31. The appearance of DNase I hypersensitive sites at the 5' end of the late SV40 genes is correlated with the transcriptional switch.
    Nucleic Acids Res. 1981 Nov 25;9(22):5949-64 PMID: 6273814
  32. The SV40 72 base repair repeat has a striking effect on gene expression both in SV40 and other chimeric recombinants.
    Nucleic Acids Res. 1981 Nov 25;9(22):6047-68 PMID: 6273820
  33. A small segment of polyoma virus DNA enhances the expression of a cloned beta-globin gene over a distance of 1400 base pairs.
    Nucleic Acids Res. 1981 Dec 11;9(23):6251-64 PMID: 6275354
  34. Expression of a beta-globin gene is enhanced by remote SV40 DNA sequences.
    Cell. 1981 Dec;27(2 Pt 1):299-308 PMID: 6277502
  35. Host-specific activation of transcription by tandem repeats from simian virus 40 and Moloney murine sarcoma virus.
    Proc Natl Acad Sci U S A. 1982 Nov;79(21):6453-7 PMID: 6292901
  36. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  37. The SV40 72 bp repeat preferentially potentiates transcription starting from proximal natural or substitute promoter elements.
    Cell. 1983 Feb;32(2):503-14 PMID: 6297796
  38. A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene.
    Cell. 1983 Jul;33(3):717-28 PMID: 6409417
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.22.8065
Published
1985-11-25
Pages
8065-81
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322110
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/13.22.8065 PMC Full Text View journal details
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