Genomic organization of human lactate dehydrogenase-A gene.

The Biochemical journal ·Vol. 231 ·No. 3 ·1985-11-01 ·Pages 537-41

Chung FZ, Tsujibo H, Bhattacharyya U, Sharief FS, LI SS

Abstract

A human genomic clone containing the lactate dehydrogenase-A (LDH-A) gene of approx. 12 kilobases in length was isolated and characterized. The protein-coding sequence is interrupted by six introns, and the positions of these introns are at the random coil regions or near the ends of secondary structures located on the surface of the LDH-A molecule. An additional intron is present at 24 nucleotides 5' to the translation initiation codon ATG, while the 3' untranslated sequence of 565 nucleotides is not interrupted. The genomic blot analysis of human placenta DNA indicates the presence of multiple LDH-A gene-related sequences.

MeSH Terms

Base Sequence DNA Restriction Enzymes Electrophoresis, Agar Gel Genotype Humans Isoenzymes L-Lactate Dehydrogenase/genetics

Chemicals

Isoenzymes L-Lactate Dehydrogenase DNA Restriction Enzymes

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Chung F Z

Tsujibo H

Bhattacharyya U

Sharief F S

LI S S

References (27)

27 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2310537

Published

1985-11-01

Pages

537-41

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1152784

Subset

Databases

GENBANK

X03077, X03078, X03079, X03080, X03081, X03082, X03083

External Links

PubMed source DOI: 10.1042/bj2310537 PMC Full Text View journal details

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Genomic organization of human lactate dehydrogenase-A gene.

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