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PMID: 3001022 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of lon mutants in Salmonella typhimurium.

Journal of bacteriology ·Vol. 165 ·No. 1 ·1986-01-00 ·Pages 193-7

Downs D, Waxman L, Goldberg AL, Roth J

Abstract

In this paper we report the isolation and characterization of lon mutants in Salmonella typhimurium. The mutants were isolated by using positive selection by chlorpromazine resistance. The physiological and biochemical properties of the lon mutants in S. typhimurium are very similar to those of Escherichia coli lon mutants. Mutants altered at this locus contain little or no activity of the ATP-dependent protease La and show a number of pleiotropic phenotypes, including increased production of capsular polysaccharides, increased sensitivity to UV light and other DNA-damaging agents, and a decreased ability to degrade abnormal proteins.

MeSH Terms
ATP-Dependent Proteases DNA Transposable Elements Endopeptidases/analysis,genetics Escherichia coli Proteins Genetic Complementation Test Heat-Shock Proteins Mutation Protease La Puromycin/metabolism Salmonella typhimurium/enzymology,genetics,radiation effects Serine Endopeptidases Ultraviolet Rays
Chemicals
DNA Transposable Elements Escherichia coli Proteins Heat-Shock Proteins Puromycin Endopeptidases ATP-Dependent Proteases Serine Endopeptidases Lon protein, E coli Protease La
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Downs D
Waxman L
Goldberg A L
Roth J
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30 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.165.1.193-197.1986
Published
1986-01-00
Pages
193-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214388
Subset
IM
Grants
NIGMS NIH HHS · GM23408 · United States
External Links
PubMed source DOI: 10.1128/jb.165.1.193-197.1986 PMC Full Text View journal details
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