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PMID: 3001358 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Truncated forms of the polyomavirus middle T antigen can substitute for the small T antigen in lytic infection.

Journal of virology ·Vol. 57 ·No. 1 ·1986-01-00 ·Pages 367-70

Templeton D, Simon S, Eckhart W

Abstract

Cloned polyomavirus genomes encoding the small T antigen or truncated forms of the middle T antigen facilitated the growth of genomes encoding only the large T antigen in mouse 3T6 cells. We conclude that an N-terminal domain of the middle T antigen, in the appropriate cellular location, can substitute for the small T antigen during lytic infection.

MeSH Terms
Animals Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/genetics,physiology Cell Compartmentation Cell Line Cell Transformation, Viral Cloning, Molecular Cytopathogenic Effect, Viral DNA, Viral/genetics Mice Oncogene Proteins, Viral/genetics,physiology Peptide Fragments/genetics,physiology Polyomavirus/genetics,physiology Recombinant Proteins/physiology Transfection
Chemicals
Antigens, Polyomavirus Transforming Antigens, Viral, Tumor DNA, Viral Oncogene Proteins, Viral Peptide Fragments Recombinant Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Templeton D
Simon S
Eckhart W
References (11)
11 references, click to expand
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    Mol Cell Biol. 1984 May;4(5):817-21 PMID: 6328268
  7. Mutation causing premature termination of the polyoma virus medium T antigen blocks cell transformation.
    J Virol. 1982 Mar;41(3):1014-24 PMID: 6284958
  8. A polyoma mutant that encodes small T antigen but not middle T antigen demonstrates uncoupling of cell surface and cytoskeletal changes associated with cell transformation.
    Mol Cell Biol. 1984 Dec;4(12):2774-83 PMID: 6098822
  9. The hr-t gene of polyoma virus.
    Biochim Biophys Acta. 1982 Dec 21;695(2):69-95 PMID: 6305416
  10. Characterization of viable mutants of polyomavirus cold sensitive for maintenance of cell transformation.
    J Virol. 1984 Mar;49(3):799-805 PMID: 6321764
  11. Transformation of rat cells by an altered polyoma virus genome expressing only the middle-T protein.
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.57.1.367-370.1986
Published
1986-01-00
Pages
367-70
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC252738
Subset
IM
Grants
NCI NIH HHS · CA-13884 · United States
NCI NIH HHS · CA-14195 · United States
NIGMS NIH HHS · GM-07198 · United States
External Links
PubMed source DOI: 10.1128/JVI.57.1.367-370.1986 PMC Full Text View journal details
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