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PMID: 3001700 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Epidermal growth factor regulates the expression of its own receptor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 24 ·1985-12-00 ·Pages 8374-8

Clark AJ, Ishii S, Richert N, Merlino GT, Pastan I

Abstract

The epidermal growth factor (EGF) receptor gene is the cellular homolog of the avian erythroblastosis virus erbB oncogene. Control of EGF receptor expression determines cellular responsiveness to EGF and might play an important role in neoplastic development. Using RNA blot hybridization, we have found that exposure of human KB carcinoma cells to EGF results in elevated levels of EGF receptor mRNA. The phorbol ester 4 beta-phorbol 12-myristate 13-acetate also stimulates EGF receptor RNA accumulation. Immunoprecipitation of metabolically labeled (30 min) EGF receptor protein revealed that synthesis of new EGF receptor follows the increase in receptor RNA. Addition of cycloheximide together with EGF further enhances EGF receptor RNA accumulation. Results of nuclear runoff-transcription experiments suggest that the stimulatory effects of EGF and cycloheximide are most likely due to a posttranscriptional control mechanism.

MeSH Terms
Cells, Cultured Cycloheximide/pharmacology Epidermal Growth Factor/physiology ErbB Receptors Gene Expression Regulation/drug effects Humans Kinetics Phorbols/pharmacology RNA, Messenger/metabolism Receptors, Cell Surface/genetics Tetradecanoylphorbol Acetate/pharmacology
Chemicals
Phorbols RNA, Messenger Receptors, Cell Surface Epidermal Growth Factor Cycloheximide ErbB Receptors Tetradecanoylphorbol Acetate
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Clark A J
Ishii S
Richert N
Merlino G T
Pastan I
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39 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.24.8374
Published
1985-12-00
Pages
8374-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390918
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.24.8374 PMC Full Text View journal details
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