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PMID: 3001702 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Specific regions of the intervening sequences of beta-globin RNA are resistant to nuclease in 50S heterogeneous nuclear RNA-protein complexes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 24 ·1985-12-00 ·Pages 8414-8

Patton JR, Chae CB

Abstract

The specific assembly of heterogeneous nuclear RNA-protein complexes (hnRNPs) containing precursor beta-globin RNA was investigated by using the 50S hnRNP released from chicken reticulocyte nuclei by endogenous nuclease. The nuclease-resistant regions were mapped on adult beta-globin intervening sequences (IVS) at the resolution of nucleotides with an RNA mapping method [Patton, J. R. and Chae, C.-B. (1983) J. Biol. Chem. 258, 3991-3995]. We found that there is one 28-nucleotide-long nuclease-resistant region in the first IVS and there are four nuclease-resistant regions in the second IVS. Of particular interest is the presence in 50S hnRNP of a nuclease-resistant region (24-28 nucleotides long) in both IVS immediately upstream from the putative lariat branch site in an RNA splicing intermediate. Our results demonstrate that hnRNPs containing precursor beta-globin RNA are, like those containing mature beta-globin RNA, assembled in a site-specific manner.

MeSH Terms
Animals Base Sequence Chickens Endonucleases/metabolism Globins Heterogeneous-Nuclear Ribonucleoproteins Macromolecular Substances Micrococcal Nuclease/metabolism RNA Splicing RNA, Double-Stranded/metabolism RNA, Heterogeneous Nuclear/metabolism Ribonucleoproteins/metabolism
Chemicals
Heterogeneous-Nuclear Ribonucleoproteins Macromolecular Substances RNA, Double-Stranded RNA, Heterogeneous Nuclear Ribonucleoproteins Globins Endonucleases Micrococcal Nuclease
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Patton J R
Chae C B
References (36)
36 references, click to expand
  1. Structural organization of nuclear complexes containing DNA-like RNA.
    J Mol Biol. 1968 Apr 14;33(1):251-63 PMID: 5646646
  2. Arrangement of 30S heterogeneous nuclear ribonucleoprotein on polyoma virus late nuclear transcripts.
    Mol Cell Biol. 1981 Jan;1(1):21-34 PMID: 6100958
  3. Some properties of RNA:protein complexes from the nucleus of eukaryotic cells.
    Cold Spring Harb Symp Quant Biol. 1974;38:921-32 PMID: 4364788
  4. The structure of chromosome-derived ribonucleoprotein in oocytes of Triturus cristatus carnifex (Laurenti).
    Chromosoma. 1974;48(2):137-58 PMID: 4457289
  5. Ultrastructural patterns of RNA synthesis during early embryogenesis of Drosophila melanogaster.
    Cell. 1976 Jun;8(2):305-19 PMID: 822943
  6. Characterization of the non-histone nuclear proteins associated with rapidly labeled heterogeneous nuclear RNA.
    J Biol Chem. 1977 Oct 25;252(20):7307-22 PMID: 903364
  7. Rearrangements in the course of ribonuclease hydrolysis of pre-messenger ribonucleoproteins. A warning.
    Eur J Biochem. 1979 Apr;95(3):593-606 PMID: 446484
  8. Post-replicative nonribosomal transcription units in D. melanogaster embryos.
    Cell. 1979 Jul;17(3):551-63 PMID: 113103
  9. Characterization of active transcription units in Balbiani rings of Chironomus tentans.
    Cell. 1979 Aug;17(4):835-48 PMID: 290410
  10. Are snRNPs involved in splicing?
    Nature. 1980 Jan 10;283(5743):220-4 PMID: 7350545
  11. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  12. Ribonucleoprotein structure in nascent hnRNA is nonrandom and sequence-dependent.
    Cell. 1980 May;20(1):75-84 PMID: 6771017
  13. Ribonucleoprotein organization of eukaryotic RNA. XV. Different nucleoprotein structures of globin messenger RNA sequences in nuclear and polyribosomal ribonucleoprotein particles.
    J Mol Biol. 1981 Aug 25;150(4):509-24 PMID: 7328643
  14. Association of globin ribonucleic acid and its precursors with the chicken erythroblast nuclear matrix.
    Biochemistry. 1982 Feb 16;21(4):764-71 PMID: 6176264
  15. Mild nuclease treatment as a probe for a non-random distribution of adenovirus-specific RNA sequences and of cellular RNA in nuclear ribonucleoprotein fibrils.
    J Mol Biol. 1982 Mar 5;155(3):185-205 PMID: 6281447
  16. Non-random localization of ribonucleoprotein (RNP) structures within an adenovirus mRNA precursor.
    Nucleic Acids Res. 1982 May 25;10(10):3053-68 PMID: 6285286
  17. A method for isolation of a large amount of a single-stranded DNA fragment.
    Anal Biochem. 1982 Oct;126(1):231-4 PMID: 6295210
  18. Analysis of the adult chicken beta-globin gene. Nucleotide sequence of the locus, microheterogeneity at the 5'-end of beta-globin mRNA, and aberrant nuclear RNA species.
    J Biol Chem. 1983 Mar 25;258(6):3983-90 PMID: 6833240
  19. A method for mapping RNA initiation, termination, splice, and protein binding sites. Ribosome binding sites on beta-globin messenger RNA.
    J Biol Chem. 1983 Mar 25;258(6):3991-5 PMID: 6300066
  20. Structure of nuclear ribonucleoprotein: heterogeneous nuclear RNA is complexed with a major sextet of proteins in vivo.
    Proc Natl Acad Sci U S A. 1983 Mar;80(6):1599-602 PMID: 6572923
  21. Evidence for an intron-contained sequence required for the splicing of yeast RNA polymerase II transcripts.
    Cell. 1983 Jun;33(2):519-27 PMID: 6305513
  22. Evidence for the biochemical role of an internal sequence in yeast nuclear mRNA introns: implications for U1 RNA and metazoan mRNA splicing.
    Cell. 1983 Sep;34(2):395-403 PMID: 6616616
  23. Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein.
    Cell. 1983 Nov;35(1):101-7 PMID: 6194895
  24. Interaction of snRNAs with rapidly sedimenting nuclear sub-structures (hnRNPs) from HeLa cells.
    Nucleic Acids Res. 1983 Oct 11;11(19):6631-46 PMID: 6195594
  25. Different RNA patterns of globin and non-globin 40S heterogeneous nuclear RNA-protein complexes in chicken reticulocyte nuclei.
    Nucleic Acids Res. 1983 Oct 25;11(20):7057-68 PMID: 6634409
  26. The 5' terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors.
    Cell. 1984 Aug;38(1):299-307 PMID: 6235919
  27. Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro.
    Cell. 1984 Aug;38(1):317-31 PMID: 6088074
  28. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  29. Intron splicing: a conserved internal signal in introns of animal pre-mRNAs.
    Proc Natl Acad Sci U S A. 1984 Dec;81(23):7417-20 PMID: 6209716
  30. Isolation of the heterogeneous nuclear RNA-ribonucleoprotein complex (hnRNP): a unique supramolecular assembly.
    Proc Natl Acad Sci U S A. 1984 Dec;81(23):7471-5 PMID: 6594697
  31. In vivo splicing products of the rabbit beta-globin pre-mRNA.
    Cell. 1984 Dec;39(3 Pt 2):589-602 PMID: 6096012
  32. Characterization of the branch site in lariat RNAs produced by splicing of mRNA precursors.
    Nature. 1985 Feb 14-20;313(6003):552-7 PMID: 2578627
  33. A role for branchpoints in splicing in vivo.
    Nature. 1985 May 30-Jun 5;315(6018):430-2 PMID: 4000270
  34. The "spliceosome": yeast pre-messenger RNA associates with a 40S complex in a splicing-dependent reaction.
    Science. 1985 May 24;228(4702):963-7 PMID: 3890181
  35. Specific regions of beta-globin RNA are resistant to nuclease digestion in RNA-protein complexes in chicken reticulocyte nuclei.
    Mol Cell Biol. 1985 Jun;5(6):1220-8 PMID: 4033649
  36. Proteins associated with heterogeneous nuclear RNA in eukaryotic cells.
    J Mol Biol. 1974 Feb 25;83(2):163-83 PMID: 4821869
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.24.8414
Published
1985-12-00
Pages
8414-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390926
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.24.8414 PMC Full Text View journal details
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