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PMID: 3003388 Published · ppublish English Journal Article

Human papillomavirus type 16 DNA-induced malignant transformation of NIH 3T3 cells.

Journal of virology ·Vol. 57 ·No. 2 ·1986-02-00 ·Pages 572-7

Yasumoto S, Burkhardt AL, Doniger J, DiPaolo JA

Abstract

A biological function for human papillomavirus 16 (HPV 16) DNA was demonstrated by transformation of NIH 3T3 cells. HPV 16 DNA has been found frequently in genital cancer and has been classified as a papillomavirus on the basis of DNA homology. A recombinant HPV 16 DNA (pSHPV16d), which contains a head-to-tail dimer of the full-length HPV 16 genome, induced morphologic transformation; the transformed cells were tumorigenic in nude mice. Expression of transforming activity was unique because of the long latency period (more than 4 weeks) required for induction of morphologic transformation and because the transfected DNA existed primarily in a multimeric form with some rearrangements. Furthermore, virus-specific RNAs were expressed in the transformants. The transformation of NIH 3T3 cells provides a model for analyzing the functions of HPV 16, which is associated with cervical carcinomas.

MeSH Terms
Animals Cell Line Cell Transformation, Viral DNA, Viral/genetics Gene Expression Regulation Mice Papillomaviridae/genetics RNA, Viral/genetics
Chemicals
DNA, Viral RNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yasumoto S
Burkhardt A L
Doniger J
DiPaolo J A
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32 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.57.2.572-577.1986
Published
1986-02-00
Pages
572-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC252771
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.57.2.572-577.1986 PMC Full Text View journal details
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