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PMID: 3003525 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of the genes and their polypeptide products responsible for aerobactin synthesis by pColV plasmids.

Molecular & general genetics : MGG ·Vol. 201 ·No. 2 ·1985-00-00 ·Pages 204-12

Gross R, Engelbrecht F, Braun V

Abstract

Iron acquisition via aerobactin enhances the virulence of Escherichia coli. Genes that specify functions for aerobactin synthesis and iron(III)-aerobactin transport have been identified on several ColV plasmids. Previously, we cloned the locus for aerobactin synthesis from pColV-K311 and assigned to three loci termed aerA, aerB, and aerC the functions for hydroxylation of lysine, acetylation of the 6-amino group of 6-hydroxy-lysine and coupling of N-acetyl-N-hydroxy-lysine with citrate, respectively (Gross et al. 1984). In this paper we show that aerA and aerB determine polypeptides with molecular weights of 50,000 and 35,000, respectively. We identified a fourth gene designated aerD that codes for a polypeptide with a molecular weight of 60,000, and which is required for the linkage of one residue of N-acetyl-N-hydroxy-lysine to citrate. The aerC gene product completes aerobactin synthesis by coupling the second N-acetyl-N-hydroxy-lysine to the monoacylated derivative citrate. The order of the genes in the operon was found to be aerD-aerB-aerC-aerA.

MeSH Terms
Base Sequence DNA Restriction Enzymes DNA, Recombinant/metabolism Escherichia coli/genetics,pathogenicity Genes Genes, Bacterial Genotype Hydroxamic Acids/metabolism Iron Chelating Agents/metabolism Peptides/genetics Plasmids Species Specificity Virulence
Chemicals
DNA, Recombinant Hydroxamic Acids Iron Chelating Agents Peptides aerobactin DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gross R
Engelbrecht F
Braun V
References (26)
26 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00425661
Published
1985-00-00
Pages
204-12
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00425661
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