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PMID: 3003540 Published · ppublish English Journal Article

Molecular cloning and nucleotide sequence of the HU-1 gene of Escherichia coli.

Molecular & general genetics : MGG ·Vol. 201 ·No. 2 ·1985-00-00 ·Pages 360-2

Kano Y, Yoshino S, Wada M, Yokoyama K, Nobuhara M, Imamoto F

Abstract

The Escherichia coli HU-1 was cloned by use of mixed synthetic oligonucleotides (17-mer) predicted from a portion of its amino acid sequence. The amino acid sequence of the HU-1 protein deduced from the nucleotide sequence is in good agreement with the published sequence. The nucleotide sequence has a possible promoter and a typical ribosomal binding site upstream from the translational initiation codon (GUG) of the HU-1 gene.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Cloning, Molecular Codon DNA Restriction Enzymes DNA-Binding Proteins/genetics Escherichia coli/genetics Genes Genes, Bacterial Plasmids
Chemicals
Bacterial Proteins Codon DNA-Binding Proteins histone-like protein HU, bacteria DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kano Y
Yoshino S
Wada M
Yokoyama K
Nobuhara M
Imamoto F
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26 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00425687
Published
1985-00-00
Pages
360-2
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00425687
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