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PMID: 3006028 Published · ppublish English Journal Article

Genes involved in Haemophilus influenzae type b capsule expression are part of an 18-kilobase tandem duplication.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 4 ·1986-02-00 ·Pages 1106-10

Hoiseth SK, Moxon ER, Silver RP

Abstract

Encapsulated Haemophilus influenzae type b produce nonencapsulated variants at high frequency (0.1-0.3%). Cosmid cloning was used to investigate the genetic mechanism responsible for this instability. Analysis of three independently derived cosmid clones showed that the b+ parental strain contains an 18-kilobase tandem duplication of genes involved in type b capsule expression. Loss of one complete copy of the 18-kilobase tandem duplication occurred following transformation of the cosmid clones into Rec+, but not Rec-, Escherichia coli, and in H. influenzae strains that had spontaneously lost capsule expression. These results suggest that high-frequency loss of type b capsule expression is due to rec-dependent recombination between the two copies of the 18-kilobase tandem repeat. This is further supported by our finding that introduction of the H. influenzae rec-1 mutation stabilized type b capsule expression.

MeSH Terms
Chromosome Mapping Chromosomes, Bacterial Cloning, Molecular Cosmids DNA Restriction Enzymes Escherichia coli/metabolism Gene Expression Regulation Genes, Bacterial Haemophilus influenzae/genetics,metabolism Polysaccharides, Bacterial/biosynthesis Rec A Recombinases/physiology Recombination, Genetic Repetitive Sequences, Nucleic Acid Transformation, Bacterial
Chemicals
Polysaccharides, Bacterial Rec A Recombinases DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hoiseth S K
Moxon E R
Silver R P
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21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.4.1106
Published
1986-02-00
Pages
1106-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323020
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.4.1106 PMC Full Text View journal details
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