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PMID: 3009878 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sequence homology between cloned caprine arthritis encephalitis virus and visna virus, two neurotropic lentiviruses.

Journal of virology ·Vol. 58 ·No. 2 ·1986-05-00 ·Pages 665-70

Pyper JM, Clements JE, Gonda MA, Narayan O

Abstract

Caprine arthritis encephalitis virus (CAEV) is an exogenous, nononcogenic retrovirus which causes neurological disease and crippling arthritis in goats. A complete CAEV genome was cloned from unintegrated viral DNA in two fragments of 9.4 and 0.4 kilobases in length, respectively. The biological activity of these clones was tested by ligation of the fragments followed by transfection onto goat synovial membrane cells; infectious virus was recovered. Cloned CAEV and visna virus, a related neurotropic virus of sheep, were compared by heteroduplex and molecular hybridization analyses. These data demonstrated that the greatest overall conservation of nucleotide sequences occurred in the gag and pol gene regions and two smaller regions, sor and the putative tat gene. The region of greatest divergence occurred in the env gene and, in particular, was localized primarily in the region coding for the glycosylated outer membrane protein. These findings and the recently demonstrated genetic relationship of visna virus, CAEV, and human T-cell lymphotropic virus type III, the etiologic agent of the acquired immune deficiency syndrome, may have important implications concerning the biological properties of these related viruses for human and veterinary medicine.

MeSH Terms
Animals Arthritis, Infectious/microbiology,veterinary Cloning, Molecular DNA/genetics DNA, Viral/genetics Encephalitis/microbiology,veterinary Gene Products, gag Genes, Viral Goats Nucleic Acid Heteroduplexes Nucleic Acid Hybridization Retroviridae/genetics Retroviridae Proteins/genetics Sequence Homology, Nucleic Acid Viral Envelope Proteins/genetics Visna-maedi virus/genetics
Chemicals
DNA, Viral Gene Products, gag Nucleic Acid Heteroduplexes Retroviridae Proteins Viral Envelope Proteins DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pyper J M
Clements J E
Gonda M A
Narayan O
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43 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.58.2.665-670.1986
Published
1986-05-00
Pages
665-70
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC252960
Subset
IM
Grants
NCI NIH HHS · N01-CO-23910 · United States
NINDS NIH HHS · NS 15721 · United States
NINDS NIH HHS · NS 16145 · United States
External Links
PubMed source DOI: 10.1128/JVI.58.2.665-670.1986 PMC Full Text View journal details
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