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PMID: 3010313 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning of the muscle gene unc-22 in Caenorhabditis elegans by Tc1 transposon tagging.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 8 ·1986-04-00 ·Pages 2579-83

Moerman DG, Benian GM, Waterston RH

Abstract

The previously described mutator system of Caenorhabditis elegans var. Bergerac has as one of its targets unc-22, a previously uncloned gene on chromosome IV important in assembly and function of the body wall musculature. By assuming that the mutator activity involved transposition of the repetitive element Tc1 into the unc-22 gene we have succeeded both in cloning the unc-22 gene and in demonstrating that Tc1 transposition is the principal basis of the mutator activity in the Bergerac strain. Although germ-line excision of Tc1 is sensitive to genetic background, somatic excision appears to be less so, suggesting that Tc1 movement is controlled differently in germ-line and somatic tissue. The availability of a transposon-based mutator system should aid in the cloning of additional genes in C. elegans, and the particular properties of this Tc1 system may provide information about the control of transposable element activity more generally.

MeSH Terms
Alleles Animals Caenorhabditis/genetics Chromosome Mapping Cloning, Molecular DNA Transposable Elements Genes Muscles/physiology Mutation Repetitive Sequences, Nucleic Acid
Chemicals
DNA Transposable Elements
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Moerman D G
Benian G M
Waterston R H
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.8.2579
Published
1986-04-00
Pages
2579-83
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323342
Subset
IM
Grants
NIGMS NIH HHS · GM23883 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.8.2579 PMC Full Text View journal details
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