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PMID: 3011739 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Excretion of the penicillinase of an alkalophilic Bacillus sp. through the Escherichia coli outer membrane is caused by insertional activation of the kil gene in plasmid pMB9.

Journal of bacteriology ·Vol. 166 ·No. 3 ·1986-06-00 ·Pages 728-32

Kobayashi T, Kato C, Kudo T, Horikoshi K

Abstract

Most of the cloned penicillinase from alkalophilic Bacillus sp. strain 170 and alkaline phosphatase were released into the culture medium by Escherichia coli strains bearing plasmid pEAP1 or pEAP2 (T. Kudo, C. Kato, and K. Horikoshi, J. Bacteriol. 156:949-951, 1983). We analyzed the basis for excretion of periplasmic enzymes in the cells bearing these plasmids. Several experiments such as subcloning, insertion of a chloramphenicol acetyltransferase cartridge, and DNA sequencing were done. A dormant kil gene in plasmid pMB9 was expressed by a promoter of the inserted DNA fragment of alkalophilic Bacillus sp. strain 170, and as a result, the outer membrane of E. coli became permeable, allowing the proteins to be excreted without cell lysis.

MeSH Terms
Amino Acid Sequence Bacillus/enzymology,genetics Bacterial Outer Membrane Proteins/metabolism Cloning, Molecular DNA Transposable Elements Escherichia coli/enzymology,genetics Genes, Bacterial Mutation Penicillinase/metabolism Plasmids
Chemicals
Bacterial Outer Membrane Proteins DNA Transposable Elements Penicillinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kobayashi T
Kato C
Kudo T
Horikoshi K
References (22)
22 references, click to expand
  1. Rapid fixed-time assay for penicillinase.
    J Bacteriol. 1968 Apr;95(4):1493-4 PMID: 5646633
  2. The ins and outs of colicins. Part I: Production, and translocation across membranes.
    Microbiol Sci. 1984 Oct;1(7):168-75 PMID: 6444117
  3. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  4. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  5. Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.
    Gene. 1977;2(2):75-93 PMID: 344136
  6. Iodometric assay method for beta-lactamase with various beta-lactam antibiotics as substrates.
    Antimicrob Agents Chemother. 1978 Jun;13(6):910-3 PMID: 677858
  7. Nucleotide sequence of small ColE1 derivatives: structure of the regions essential for autonomous replication and colicin E1 immunity.
    Mol Gen Genet. 1979 May 4;172(2):151-9 PMID: 384144
  8. DNA sequence of the gene for the outer membrane lipoprotein of E. coli: an extremely AT-rich promoter.
    Cell. 1979 Dec;18(4):1109-17 PMID: 391404
  9. Protein H encoded by plasmid Clo DF13 involved in lysis of the bacterial host. I. Localisation of the gene and identification and subcellular localisation of the gene H product.
    Mol Gen Genet. 1981;183(2):318-25 PMID: 6276683
  10. Protein H encoded by plasmid CloDF13 is involved in excretion of cloacin DF13.
    J Bacteriol. 1982 Jun;150(3):1115-21 PMID: 6281236
  11. cea-kil operon of the ColE1 plasmid.
    J Bacteriol. 1983 Mar;153(3):1479-85 PMID: 6298187
  12. Construction and characterization of the chloramphenicol-resistance gene cartridge: a new approach to the transcriptional mapping of extrachromosomal elements.
    Gene. 1982 Dec;20(2):305-16 PMID: 6299895
  13. Cyclic AMP-dependent initiation and rho-dependent termination of colicin E1 gene transcription.
    J Biol Chem. 1983 Jun 10;258(11):7072-8 PMID: 6304076
  14. A genetic approach to the study of mitomycin-induced lysis of Escherichia coli K-12 strains which produce colicin E2.
    Mol Gen Genet. 1983;190(3):366-72 PMID: 6308394
  15. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  16. Translational block to expression of the Escherichia coli Tn9-derived chloramphenicol-resistance gene in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1982 Oct;79(19):5886-90 PMID: 6310552
  17. Developmental and genetic regulation of Bacillus subtilis genes transcribed by sigma 28-RNA polymerase.
    Cell. 1983 Nov;35(1):285-93 PMID: 6313226
  18. Excretion of the penicillinase of an alkalophilic Bacillus sp. through the Escherichia coli outer membrane.
    J Bacteriol. 1983 Nov;156(2):949-51 PMID: 6313630
  19. Factors necessary for the export process of colicin E1 across cytoplasmic membrane of Escherichia coli.
    Eur J Biochem. 1984 Apr 16;140(2):249-55 PMID: 6425060
  20. Colicin E2 release: lysis, leakage or secretion? Possible role of a phospholipase.
    EMBO J. 1984 Oct;3(10):2393-7 PMID: 6389120
  21. Cloning of a developmentally regulated element from alkalophilic Bacillus subtilis DNA.
    J Bacteriol. 1985 Jan;161(1):158-63 PMID: 2981799
  22. Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria.
    Methods Enzymol. 1975;43:737-55 PMID: 1094240
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.166.3.728-732.1986
Published
1986-06-00
Pages
728-32
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215185
Subset
IM
Databases
GENBANK
M13467, M13468
External Links
PubMed source DOI: 10.1128/jb.166.3.728-732.1986 PMC Full Text View journal details
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