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PMID: 3013616 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Regulation of the operon encoding ribonucleotide reductase in Escherichia coli: evidence for both positive and negative control.

The EMBO journal ·Vol. 5 ·No. 5 ·1986-05-00 ·Pages 1077-85

Tuggle CK, Fuchs JA

Abstract

The ribonucleotide reductase genes (nrd) are induced by thymine starvation. Deletion analysis of the sequences upstream of the cloned nrd genes was used to identify several regulatory regions. The start of transcription (nrdP) was mapped 110 bp upstream of nrdA, the first structural gene. A site required for positive regulation of nrd was mapped 135 bp upstream of nrdP in a region with two direct repeat sequences as well as potential secondary structure. Two other sites (one upstream of nrdP, the other downstream) were identified as sequences whose deletion markedly increase expression. These latter sites show sequence homology and probably interact since the effects of their individual deletion are not additive when combined.

MeSH Terms
Base Sequence DNA Restriction Enzymes Escherichia coli/enzymology,genetics Genes Genes, Bacterial Genes, Regulator Kinetics Nucleic Acid Conformation Operon Plasmids Ribonucleotide Reductases/genetics
Chemicals
Ribonucleotide Reductases DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tuggle C K
Fuchs J A
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28 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-05-00
Pages
1077-85
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1166904
Subset
IM
Grants
NIGMS NIH HHS · GM 07323 · United States
NIGMS NIH HHS · GM 20884 · United States
External Links
PubMed source PMC Full Text View journal details
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