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PMID: 3014435 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning of the STA2 and SGA genes encoding glucoamylases in yeasts and regulation of their expression by the STA10 gene of Saccharomyces cerevisiae.

Nucleic acids research ·Vol. 14 ·No. 12 ·1986-06-25 ·Pages 4701-18

Pardo JM, Polaina J, Jiménez A

Abstract

The Saccharomyces STA2 and SGA genes, encoding the extracellular and intracellular sporulation-specific glucoamylase respectively, have been cloned and their transcription and regulation studied. The STA2 gene differs from the SGA gene in that it contains an extra piece of DNA, which encodes the domain for exportation of the extracellular glucoamylase. The STA2 gene produces a single 2.85 kb transcript. Transcription of the SGA gene is initiated from two different sites, yielding two transcripts of 1.95 and 2.40 kb. Transcription of both STA2 and SGA genes is repressed by the STA10 gene of Saccharomyces cerevisiae.

MeSH Terms
Chromosome Mapping Cloning, Molecular DNA Restriction Enzymes Endonucleases Gene Expression Regulation Genes, Fungal Genes, Regulator Glucan 1,4-alpha-Glucosidase/genetics Glucosidases/genetics Plasmids RNA, Messenger/genetics Saccharomyces cerevisiae/enzymology,genetics Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic
Chemicals
RNA, Messenger Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases Glucosidases Glucan 1,4-alpha-Glucosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pardo J M
Polaina J
Jiménez A
References (16)
16 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.12.4701
Published
1986-06-25
Pages
4701-18
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311485
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/14.12.4701 PMC Full Text View journal details
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