Home LiteratureArticle Details
PMID: 301569 Published · ppublish English Journal Article

Relaxation experiments using bath-applied suberyldicholine.

The Journal of physiology ·Vol. 268 ·No. 2 ·1977-06-00 ·Pages 271-89

Adams PR

Abstract

1. The effect of step changes in membrane potential on the end-plate conductance change produced by bath-applied suberyldicholine was studied in voltage-clamped frog muscle fibres. 2. The suberyldicholine-induced conductance increased exponentially from its previous equilibrium level to a new equilibrium level following a step hyperpolarization. 3. For low suberyldicholine concentrations the time constant of this relaxation was independent of the concentration. 4. For low suberyldicholine concentrations the voltage dependence of equilibrium conductance and relaxation time constants was identical. 5. Bungarotoxin pretreatment did not affect the responses beyond a simple reduction in their amplitude. 6. The conductance evoked by high suberyldicholine concentrations was less voltage-sensitive than that evoked by low concentrations. 7. A new model for explaining noise and relaxation data is proposed. This postulates rate-limiting binding steps followed by a voltage-dependent isomerization.

MeSH Terms
Animals Anura Bungarotoxins/pharmacology Choline/analogs & derivatives,pharmacology Dicarboxylic Acids/pharmacology Dose-Response Relationship, Drug Electric Conductivity In Vitro Techniques Kinetics Membrane Potentials/drug effects Models, Neurological Motor Endplate/drug effects,physiology Muscle Contraction/drug effects Muscle Relaxation/drug effects Neostigmine/pharmacology Rana esculenta Rana temporaria
Chemicals
Bungarotoxins Dicarboxylic Acids subecholine Neostigmine Choline
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Adams P R
References (34)
34 references, click to expand
  1. ON THE NATURE OF ALLOSTERIC TRANSITIONS: A PLAUSIBLE MODEL.
    J Mol Biol. 1965 May;12:88-118 PMID: 14343300
  2. A study of the desensitization produced by acetylcholine at the motor end-plate.
    J Physiol. 1957 Aug 29;138(1):63-80 PMID: 13463799
  3. Measurement of current-voltage relations in the membrane of the giant axon of Loligo.
    J Physiol. 1952 Apr;116(4):424-48 PMID: 14946712
  4. Noise analysis of drug induced voltage clamp currents in denervated frog muscle fibres.
    J Physiol. 1976 Jul;258(3):705-29 PMID: 1086359
  5. Junctional and extrajunctional acetylcholine receptors in normal and denervated frog muscle fibres. Noise analysis experiments with different agonists.
    Pflugers Arch. 1976 Oct 15;366(1):1-9 PMID: 185583
  6. The statistical nature of the acetycholine potential and its molecular components.
    J Physiol. 1972 Aug;224(3):665-99 PMID: 5071933
  7. A quantitative description of end-plate currents.
    J Physiol. 1972 May;223(1):173-97 PMID: 5046143
  8. Voltage clamp analysis of acetylcholine produced end-plate current fluctuations at frog neuromuscular junction.
    J Physiol. 1973 Dec;235(3):655-91 PMID: 4543940
  9. The characteristics of 'end-plate noise' produced by different depolarizing drugs.
    J Physiol. 1973 May;230(3):707-17 PMID: 4717155
  10. The binding of acetylcholine to receptors and its removal from the synaptic cleft.
    J Physiol. 1973 Jun;231(3):549-74 PMID: 4361216
  11. Kinetics of allosteric enzymes.
    Annu Rev Biophys Bioeng. 1974;3(0):1-33 PMID: 4371650
  12. Post-synaptic potentiation: interaction between quanta of acetylcholine at the skeletal neuromuscular synapse.
    J Physiol. 1975 Oct;251(2):427-63 PMID: 171379
  13. Voltage dependence of agonist effectiveness at the frog neuromuscular junction: resolution of a paradox.
    J Physiol. 1975 Oct;251(2):245-70 PMID: 1081139
  14. The kinetics of action of acetylcholine antagonists in smooth muscle.
    Proc R Soc Lond B Biol Sci. 1966 Apr 19;164(996):488-510 PMID: 4379580
  15. Influence of chloride ions on changes in membrane potential during prolonged application of carbachol to frog skeletal muscle.
    Br J Pharmacol. 1973 Feb;47(2):363-76 PMID: 4541779
  16. Mechanisms of drug action at the voluntary muscle endplate.
    Annu Rev Pharmacol. 1975;15:307-25 PMID: 238463
  17. The effect of alpha-bungarotoxin on acetylcholine receptors.
    Br J Pharmacol. 1973 Sep;49(1):138-9 PMID: 4787522
  18. Allosteric mechanisms at neuromuscular junctions.
    Neurosci Res Program Bull. 1973 Jun;11(3):220-4 PMID: 4736777
  19. Drug receptors and their function.
    Nature. 1971 May 14;231(5298):91-6 PMID: 4930103
  20. A study of desensitization using voltage clamp.
    Pflugers Arch. 1975 Oct 28;360(2):135-44 PMID: 1081218
  21. Single-channel currents recorded from membrane of denervated frog muscle fibres.
    Nature. 1976 Apr 29;260(5554):799-802 PMID: 1083489
  22. Conductance of channels opened by acetylcholine-like drugs in muscle end-plate.
    Nature. 1975 Jan 17;253(5488):204-6 PMID: 1078602
  23. Effects of membrane potential on the capacitance of skeletal muscle fibers.
    J Gen Physiol. 1976 Feb;67(2):125-63 PMID: 1082924
  24. Acetylcholine receptor: modification of synaptic gating mechanism after treatment with a disulfide bond reducing agent.
    Pflugers Arch. 1975 Mar 22;355(1):19-26 PMID: 1080270
  25. Voltage-dependence of drug-induced conductance in frog neuromuscular junction.
    Proc Natl Acad Sci U S A. 1975 Jun;72(6):2140-4 PMID: 1079601
  26. Kinetics of agonist conductance changes during hyperolarization at frog endplates.
    Br J Pharmacol. 1975 Feb;53(2):308-10 PMID: 1080064
  27. Relaxation measurements on the acetylcholine receptor.
    Proc Natl Acad Sci U S A. 1975 Sep;72(9):3496-500 PMID: 1059136
  28. Current-voltage relation and reversal potential at junctional and extrajunctional ACh-receptors of the frog neuromuscular junction.
    Pflugers Arch. 1976 Mar 11;362(1):43-7 PMID: 943777
  29. Voltage dependence of agonist responses at voltage-clamped frog endplates.
    Pflugers Arch. 1976 Jan 30;361(2):145-51 PMID: 943088
  30. An analysis of the dose-response curve at voltage-clamped frog-endplates.
    Pflugers Arch. 1975 Oct 28;360(2):145-53 PMID: 1237869
  31. An extended kinetic analysis of valinomycin-induced Rb-transport through monoglyceride membranes.
    J Membr Biol. 1975-1976;25(3-4):249-70 PMID: 1235803
  32. Conductance increases produced by bath application of cholinergic agonists to Electrophorus electroplaques.
    J Gen Physiol. 1975 Jun;65(6):797-816 PMID: 1194885
  33. Density and dose-response curve of acetylcholine receptors in frog neuromuscular junction.
    Nature. 1975 Feb 20;253(5493):641-3 PMID: 1113858
  34. Voltage clamp on Helix pomatia neuronal membrane; current measurement over a limited area of the soma surface.
    Pflugers Arch. 1969;311(3):272-7 PMID: 5388560
Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1977.sp011857
Published
1977-06-00
Pages
271-89
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1283664
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.1977.sp011857 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2