Home LiteratureArticle Details
PMID: 3015891 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Electron-paramagnetic-resonance spectroscopy of Bacillus subtilis cytochrome b558 in Escherichia coli membranes and in succinate dehydrogenase complex from Bacillus subtilis membranes.

Journal of bacteriology ·Vol. 167 ·No. 2 ·1986-08-00 ·Pages 735-9

Hederstedt L, Andersson KK

Abstract

Cytochrome b558 of the Bacillus subtilis succinate dehydrogenase complex was studied by electron-paramagnetic-resonance (EPR) spectroscopy. The cytochrome amplified in Escherichia coli membranes by expression of the cloned cytochrome gene and in the succinate dehydrogenase complex immunoprecipitated from solubilized B. subtilis membranes, respectively, is shown to be low spin with a highly anisotropic (gmax approximately equal to 3.5) EPR signal. The amino acid residues most likely forming fifth and sixth axial ligands to heme in cytochrome b558 are discussed on the basis of the EPR signal and the recently determined gene sequence (K. Magnusson, M. Philips, J.R. Guest, and L. Rutberg, J. Bacteriol. 166:1067-1071, 1986) and in comparison with other b-type cytochromes.

MeSH Terms
Bacillus subtilis Cell Membrane/enzymology,ultrastructure Cytochrome b Group Electron Spin Resonance Spectroscopy Escherichia coli Heme NADPH Oxidases Succinate Dehydrogenase
Chemicals
Cytochrome b Group Heme cytochrome b558 Succinate Dehydrogenase NADPH Oxidases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hederstedt L
Andersson K K
References (30)
30 references, click to expand
  1. Biosynthesis and membrane binding of succinate dehydrogenase in Bacillus subtilis.
    J Bacteriol. 1980 Dec;144(3):941-51 PMID: 6777371
  2. Study of the respiratory chain in Micrococcus luteus (lysodeikticus) by electron-spin-resonance spectroscopy.
    Eur J Biochem. 1983 Dec 1;137(1-2):185-90 PMID: 6317382
  3. Succinate dehydrogenase--a comparative review.
    Microbiol Rev. 1981 Dec;45(4):542-55 PMID: 6799760
  4. Purification and characterization of highly purified cytochrome b from complex III of baker's yeast.
    Biochim Biophys Acta. 1982 Sep 15;681(3):484-95 PMID: 6289886
  5. Orientation of succinate dehydrogenase and cytochrome b558 in the Bacillus subtilis cytoplasmic membrane.
    J Bacteriol. 1983 Jan;153(1):57-65 PMID: 6401289
  6. Molecular properties of succinate dehydrogenase isolated from Micrococcus luteus (lysodeikticus).
    J Bacteriol. 1983 Mar;153(3):1493-501 PMID: 6402500
  7. Characterization of a pleiotropic succinate dehydrogenase-negative mutant of Bacillus subtilis.
    J Gen Microbiol. 1983 Apr;129(4):917-22 PMID: 6411859
  8. Location of haem-binding sites in the mitochondrial cytochrome b.
    FEBS Lett. 1984 Jan 30;166(2):367-72 PMID: 6363134
  9. Cytochrome electron spin resonance line shapes, ligand fields, and components stoichiometry in ubiquinol-cytochrome c oxidoreductase.
    J Biol Chem. 1984 Feb 25;259(4):2331-6 PMID: 6321467
  10. Sequence homology and structural similarity between cytochrome b of mitochondrial complex III and the chloroplast b6-f complex: position of the cytochrome b hemes in the membrane.
    Proc Natl Acad Sci U S A. 1984 Feb;81(3):674-8 PMID: 6322162
  11. Characterization by electron paramagnetic resonance and studies on subunit location and assembly of the iron-sulfur clusters of Bacillus subtilis succinate dehydrogenase.
    J Biol Chem. 1985 May 10;260(9):5554-62 PMID: 2985599
  12. Cloning and expression in Escherichia coli of sdhA, the structural gene for cytochrome b558 of the Bacillus subtilis succinate dehydrogenase complex.
    J Bacteriol. 1985 Jun;162(3):1180-5 PMID: 2987185
  13. EPR studies of the cytochrome b-c 1 segment of the mitochondrial electron transfer system.
    Biochem Biophys Res Commun. 1971 Nov;45(4):871-8 PMID: 4330143
  14. Changes in terminal respiratory pathways of Bacillus subtilis during germination, outgrowth and vegetative growth.
    J Bacteriol. 1971 Nov;108(2):652-61 PMID: 4331498
  15. Electron paramagnetic resonance-detectable electron acceptors in beef heart mitochondria. Ubihydroquinone-cytochrome c reductase segment of the electron transfer system and complex mitochondrial fragments.
    J Biol Chem. 1974 Mar 25;249(6):1928-39 PMID: 4361833
  16. Thermodynamic and EPR characterization of mitochondrial succinate-cytochrome c reductase-phospholipid complexes.
    Biochim Biophys Acta. 1975 Apr 14;387(1):95-106 PMID: 236028
  17. Multiple low spin forms of the cytochrome c ferrihemochrome. EPR spectra of various eukaryotic and prokaryotic cytochromes c.
    J Biol Chem. 1977 Jan 25;252(2):574-82 PMID: 13072
  18. Characterization of a succinate dehydrogenase complex solubilized from the cytoplasmic membrane of Bacillus subtilis with the nonionic detergent Triton X-100.
    J Bacteriol. 1979 May;138(2):370-6 PMID: 108258
  19. Role of heme in synthesis and membrane binding of succinic dehydrogenase in Bacillus subtilis.
    J Bacteriol. 1979 May;138(2):377-82 PMID: 108259
  20. The iron-sulphur centres of aerobically-grown Escherichia coli K12.
    FEBS Lett. 1980 Feb 25;111(1):223-7 PMID: 6244182
  21. An EPR study of cytochromes from spinach chloroplasts.
    FEBS Lett. 1980 Feb 25;111(1):228-31 PMID: 6244183
  22. Cytochrome b reducible by succinate in an isolated succinate dehydrogenase-cytochrome b complex from Bacillus subtilis membranes.
    J Bacteriol. 1980 Dec;144(3):933-40 PMID: 6777370
  23. The succinate dehydrogenase of Escherichia coli. Immunochemical resolution and biophysical characterization of a 4-subunit enzyme complex.
    J Biol Chem. 1985 Aug 5;260(16):9427-34 PMID: 2991245
  24. The mitochondrial electron transport and oxidative phosphorylation system.
    Annu Rev Biochem. 1985;54:1015-69 PMID: 2862839
  25. The electron paramagnetic resonance of metalloproteins.
    Biochem Soc Trans. 1985 Jun;13(3):548-60 PMID: 2993061
  26. Axial ligands of chloroplast cytochrome b-559: identification and requirement for a heme-cross-linked polypeptide structure.
    Biochemistry. 1985 Jul 2;24(14):3638-45 PMID: 2994713
  27. Tetraheme cytochrome c-554 from Nitrosomonas europaea. Heme-heme interactions and ligand binding.
    J Biol Chem. 1986 Jan 25;261(3):1126-38 PMID: 3003055
  28. Nucleotide sequence of the gene for cytochrome b558 of the Bacillus subtilis succinate dehydrogenase complex.
    J Bacteriol. 1986 Jun;166(3):1067-71 PMID: 3086287
  29. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  30. The coordination environment of mitochondrial cytochromes b.
    FEBS Lett. 1981 Sep 28;132(2):243-6 PMID: 6271592
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.167.2.735-739.1986
Published
1986-08-00
Pages
735-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212955
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.167.2.735-739.1986 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2