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PMID: 3017839 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Bkm sequences are polymorphic in humans and are clustered in pericentric regions of various acrocentric chromosomes including the Y.

Human genetics ·Vol. 73 ·No. 4 ·1986-08-00 ·Pages 304-8

Singh L, Jones KW

Abstract

Probes of uncloned Bkm satellite DNA and a Drosophila clone 2 (8), consisting mainly of GATA repeats related to a major sequence component in Bkm, have been used to probe Southern blots of human male and female DNAs obtained from a Caucasian and an Australian aboriginal population and to human chromosomes in situ. Hybridization was observed to a distinct and an indistinct series of bands against a smeared background. The same distinct bands are identified in the DNA samples with both probes, but are most readily detected using the uncloned Bkm probe. Most restriction bands are common to both populations and some are polymorphic. However, certain bands appear to be characteristic of the Australian aboriginal samples. There are no distinct sex-linked patterns. However all of the small acrocentric human chromosomes, including the Y chromosome show hybridization to uncloned Bkm in situ.

MeSH Terms
Centromere DNA Restriction Enzymes DNA, Satellite/genetics Humans Nucleic Acid Hybridization Polymorphism, Genetic Repetitive Sequences, Nucleic Acid Y Chromosome
Chemicals
DNA, Satellite DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Singh L
Jones K W
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20 references, click to expand
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Article Info
Journal
Human genetics
Abbr.
Hum Genet
ISSN
0340-6717
DOI
10.1007/BF00279091
Published
1986-08-00
Pages
304-8
Language
English
Region
Germany
NLM ID
7613873
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00279091 View journal details
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