Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli.

Molecular & general genetics : MGG ·Vol. 204 ·No. 1 ·1986-07-00 ·Pages 1-7

Ishino Y, Shinagawa H, Makino K, Tsunasawa S, Sakiyama F, Nakata A

Abstract

The DNA ligase of Escherichia coli catalyses the NAD-dependent formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in DNA. It is essential for DNA replication and repair of damaged DNA strands. We determined the nucleotide sequence of the lig gene of Escherichia coli coding for DNA ligase and flanking regions. The coding frame of the gene was confirmed by the amino acid composition and the amino- and carboxyl-terminal amino acid sequences of the purified ligase. The ligase consists of 671 amino acid residues with a molecular weight of 73,690.

MeSH Terms

Amino Acid Sequence Base Sequence DNA Ligases/genetics DNA Restriction Enzymes Escherichia coli/enzymology,genetics Genes Genes, Bacterial Plasmids Polynucleotide Ligases/genetics

Chemicals

DNA Restriction Enzymes DNA Ligases Polynucleotide Ligases

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Ishino Y

Shinagawa H

Makino K

Tsunasawa S

Sakiyama F

Nakata A

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31 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00330179

Published

1986-07-00

Pages

1-7

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00330179

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Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli.

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