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PMID: 3018724 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A DNA binding protein specific for an origin of replication of herpes simplex virus type 1.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 17 ·1986-09-00 ·Pages 6322-6

Elias P, O'Donnell ME, Mocarski ES, Lehman IR

Abstract

We have identified a protein that binds specifically to an origin of replication (oris) of the herpes simplex virus type 1 genome. The oris binding protein, detectable only in nuclear extracts of infected cells, shows the same time course of appearance as the herpesvirus-induced DNA polymerase and the DNA binding protein ICP8. The partially purified oris binding protein generates a DNase I "footprint" that spans 18- of the 90-base-pair minimal oris sequence. The oris binding protein may, therefore, be analogous to other origin-specific binding proteins that are required for the initiation of viral and chromosomal DNA replication.

MeSH Terms
Base Sequence Binding Sites DNA Replication DNA-Binding Proteins/genetics Genes, Viral Simplexvirus/genetics Time Factors Viral Proteins/genetics Virus Replication
Chemicals
DNA-Binding Proteins Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Elias P
O'Donnell M E
Mocarski E S
Lehman I R
References (30)
30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.17.6322
Published
1986-09-00
Pages
6322-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386495
Subset
IM
Grants
NIAID NIH HHS · AI20211 · United States
NIGMS NIH HHS · GM 06196 · United States
Databases
GENBANK
M13830
External Links
PubMed source DOI: 10.1073/pnas.83.17.6322 PMC Full Text View journal details
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