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PMID: 3019673 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Differential regulation of papilloma virus early gene expression in transformed fibroblasts and carcinoma cell lines.

The EMBO journal ·Vol. 5 ·No. 8 ·1986-08-00 ·Pages 1945-50

Kleiner E, Dietrich W, Pfister H

Abstract

Treatment of bovine papilloma virus (BPV) 1-transformed mouse fibroblasts with cycloheximide led to a 10-fold increase in the amount of viral transcripts, after as little as 1 h of protein synthesis inhibition. Northern blots revealed no qualitative changes in the RNA pattern. Nuclear run-on experiments showed about a 7-fold increase in specific transcriptional activity after cycloheximide treatment. The half-life of BPV1 mRNA was twice as long as in untreated controls. These results indicate that both RNA synthesis and degradation of viral RNA are controlled by labile proteins. Cycloheximide stimulation turned out to be independent of the BPV1 E2 gene activity which enhances viral transcription. Cycloheximide treatment had no effect on the amount of human papilloma virus (HPV) 18 transcripts in cervical carcinoma derived HeLa and C4-1 cells. Transcription of HPV16 in the cervical carcinoma line SiHa was likewise unaffected. The differential regulation of transcription in transformed fibroblasts and cancer-derived cells, and the significance for malignant conversion are discussed.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Bovine papillomavirus 1/drug effects,genetics Cattle Cell Line Cell Transformation, Neoplastic Cycloheximide/pharmacology Embryo, Mammalian Genes, Viral Kinetics Mice Papillomaviridae/genetics Protein Biosynthesis/drug effects Transcription, Genetic
Chemicals
Cycloheximide
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kleiner E
Dietrich W
Pfister H
References (35)
35 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-08-00
Pages
1945-50
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1167062
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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