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PMID: 3019996 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Control of sensitivity to inactivation by H2O2 and broad-spectrum near-UV radiation by the Escherichia coli katF locus.

Journal of bacteriology ·Vol. 168 ·No. 1 ·1986-10-00 ·Pages 13-21

Sammartano LJ, Tuveson RW, Davenport R

Abstract

Mutations in the Escherichia coli katF gene (hydroperoxidase II) result in sensitivity to inactivation by H2O2 and broad-spectrum near-UV (NUV; 300 to 400 nm) radiation. Another mutation, nur, originally described as conferring sensitivity to inactivation by broad-spectrum and monochromatic NUV, also confers sensitivity to inactivation by H2O2. Genetic analysis via transduction suggests that the nur mutation allele of the katF locus. As previously reported for broad-spectrum and monochromatic NUV wavelengths, the sensitivity of a particular strain to H2O2 inactivation is also independent of the recA and uvrA alleles. Extracts of nur and katF strains lack catalase (hydroperoxidase II) as revealed by polyacrylamide gels stained for such activity, which is consistent with the genetic results.

MeSH Terms
Alleles DNA Repair Escherichia coli/drug effects,enzymology,genetics,radiation effects Genes, Bacterial Hydrogen Peroxide/pharmacology Isoenzymes/genetics,metabolism Mutation Peroxidase Peroxidases/genetics,metabolism Transduction, Genetic Ultraviolet Rays
Chemicals
Isoenzymes Hydrogen Peroxide Peroxidases Peroxidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sammartano L J
Tuveson R W
Davenport R
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39 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.168.1.13-21.1986
Published
1986-10-00
Pages
13-21
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213414
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.168.1.13-21.1986 PMC Full Text View journal details
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