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PMID: 3020368 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning and expression in Escherichia coli of the structural gene for the hemolytic toxin aerolysin from Aeromonas hydrophila.

Molecular & general genetics : MGG ·Vol. 204 ·No. 2 ·1986-08-00 ·Pages 289-95

Howard SP, Buckley JT

Abstract

The structural gene for the hemolytic toxin aerolysin has been cloned into the plasmid vectors pBR322 and pEMBL8+. The gene was localized on the hybrid plasmids by analysis of plasmids generated by transposon mutagenesis. The sequence of the first 683 bases of an insert in pEMBL8+ was determined and shown to encode the amino terminus of the protein as well as a typical signal sequence of 23 amino acids. Aerolysin is produced by E. coli cells containing the cloned aerolysin gene and it is processed normally by removal of the signal sequence, however it is not released from the cell. The protein appears to be translocated across the inner membrane of E. coli as its signal sequence is removed and the processed protein can be released by osmotic shock.

MeSH Terms
Aeromonas/genetics Amino Acid Sequence Bacterial Toxins/genetics Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA Transposable Elements Escherichia coli/genetics Genes Genes, Bacterial Hemolysin Proteins/genetics Plasmids Pore Forming Cytotoxic Proteins Transcription, Genetic
Chemicals
Bacterial Toxins DNA Transposable Elements Hemolysin Proteins Pore Forming Cytotoxic Proteins aerolysin DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Howard S P
Buckley J T
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00425512
Published
1986-08-00
Pages
289-95
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00425512
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