Home LiteratureArticle Details
PMID: 3022012 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Production of guanidine-resistant and -dependent poliovirus mutants from cloned cDNA: mutations in polypeptide 2C are directly responsible for altered guanidine sensitivity.

Journal of virology ·Vol. 60 ·No. 2 ·1986-11-00 ·Pages 793-6

Pincus SE, Wimmer E

Abstract

cDNA fragments representing the region in polypeptide 2C containing mutations in a guanidine-resistant or -dependent mutant were cloned into the wild-type background of an infectious clone. Transfection of COS-1 cells with these plasmids yielded viruses that were either completely resistant to 2.0 mM guanidine hydrochloride or dependent on this concentration of drug for growth.

MeSH Terms
Cell Line Cloning, Molecular DNA Drug Resistance, Microbial Genes, Viral Guanidine Guanidines/pharmacology Mutation Peptides/genetics Poliovirus/drug effects,genetics Transfection Viral Proteins/genetics
Chemicals
Guanidines Peptides Viral Proteins DNA Guanidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pincus S E
Wimmer E
References (24)
24 references, click to expand
  1. Systematic nomenclature of picornavirus proteins.
    J Virol. 1984 Jun;50(3):957-9 PMID: 6726891
  2. Guanidine-resistant poliovirus mutants produce modified 37-kilodalton proteins.
    J Virol. 1984 Jun;50(3):922-8 PMID: 6328023
  3. Recombinants of Mahoney and Sabin strain poliovirus type 1: analysis of in vitro phenotypic markers and evidence that resistance to guanidine maps in the nonstructural proteins.
    Virology. 1984 Aug;137(1):74-85 PMID: 6089422
  4. In vitro phenotypic markers of a poliovirus recombinant constructed from infectious cDNA clones of the neurovirulent Mahoney strain and the attenuated Sabin 1 strain.
    J Virol. 1985 Mar;53(3):786-92 PMID: 2983090
  5. Guanidine-selected mutants of poliovirus: mapping of point mutations to polypeptide 2C.
    J Virol. 1986 Feb;57(2):638-46 PMID: 3003395
  6. A poliovirus temperature-sensitive RNA synthesis mutant located in a noncoding region of the genome.
    Proc Natl Acad Sci U S A. 1986 Feb;83(3):571-5 PMID: 3003739
  7. Construction of poliovirus intertypic recombinants by use of cDNA.
    J Virol. 1986 Mar;57(3):1187-90 PMID: 3005620
  8. A chimeric plasmid from cDNA clones of poliovirus and coxsackievirus produces a recombinant virus that is temperature-sensitive.
    Proc Natl Acad Sci U S A. 1986 Mar;83(6):1777-81 PMID: 3006071
  9. Poliovirus mutant that does not selectively inhibit host cell protein synthesis.
    Mol Cell Biol. 1985 Nov;5(11):2913-23 PMID: 3018486
  10. Studies of the inhibitory action of guanidine on poliovirus multiplication in cell cultures.
    Virology. 1961 Sep;15:65-74 PMID: 13696674
  11. Antiviral effect of guanidine.
    Science. 1961 Aug 25;134(3478):558-9 PMID: 13741634
  12. In vitro inhibition of infectivity of polio viruses by guanidine.
    Nature. 1962 Jan 6;193:97-8 PMID: 14466152
  13. Action of guanidine on the replication of poliovirus RNA.
    Virology. 1968 Jul;35(3):408-17 PMID: 4298649
  14. Initiation of polyribosome formation in poliovirus-infected HeLa cells.
    J Mol Biol. 1970 Feb 14;47(3):275-91 PMID: 4313849
  15. Dynamics of poliovirus-specific RNA synthesis and the effects of inhibitors of virus replication.
    Virology. 1970 Mar;40(3):634-42 PMID: 4314509
  16. The role of cytoplasmic membranes in poliovirus biosynthesis.
    Virology. 1970 Sep;42(1):100-11 PMID: 4318974
  17. Structure and expression of the picornavirus genome.
    Ann N Y Acad Sci. 1980;354:183-201 PMID: 6261644
  18. Primary structure, gene organization and polypeptide expression of poliovirus RNA.
    Nature. 1981 Jun 18;291(5816):547-53 PMID: 6264310
  19. Picornaviral structure and assembly.
    Microbiol Rev. 1981 Jun;45(2):287-315 PMID: 7022155
  20. Cloned poliovirus complementary DNA is infectious in mammalian cells.
    Science. 1981 Nov 20;214(4523):916-9 PMID: 6272391
  21. Inhibition of poliovirus polymerase by guanidine in vitro.
    J Virol. 1982 Jan;41(1):313-8 PMID: 6283124
  22. Guanidine-resistant mutants of aphthovirus induce the synthesis of an altered nonstructural polypeptide, P34.
    J Virol. 1982 May;42(2):389-94 PMID: 6283162
  23. Restriction map of double-stranded DNA copy synthesized from poliovirus Sabin 1 RNA.
    J Biochem. 1982 May;91(5):1593-600 PMID: 6284728
  24. Production of infectious poliovirus from cloned cDNA is dramatically increased by SV40 transcription and replication signals.
    Nucleic Acids Res. 1984 Jun 25;12(12):5123-41 PMID: 6330698
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.60.2.793-796.1986
Published
1986-11-00
Pages
793-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC288960
Subset
IM
Grants
NIAID NIH HHS · AI-06920 · United States
NIAID NIH HHS · AI-15122 · United States
NCI NIH HHS · CA-28146 · United States
External Links
PubMed source DOI: 10.1128/JVI.60.2.793-796.1986 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2