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PMID: 3022015 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Construction and properties of a viable herpes simplex virus 1 recombinant lacking coding sequences of the alpha 47 gene.

Journal of virology ·Vol. 60 ·No. 2 ·1986-11-00 ·Pages 807-12

Mavromara-Nazos P, Ackermann M, Roizman B

Abstract

The five alpha genes, alpha 0, alpha 4, alpha 22, alpha 27, and alpha 47, are the first set of herpes simplex virus 1 genes to be transcribed and expressed in productively infected cells. We report here the construction of a viral recombinant from which all of the coding sequences of the alpha 47 gene were deleted. In addition to the alpha 47 protein, infected cell lysates did not contain detectable amounts of two polypeptide bands with apparent molecular weights of 18,000 and 21,000 which could be specified by a gene whose regulatory domain and 5' transcribed noncoding sequences overlap with the coding sequences of the alpha 47 gene. The alpha 47- virus grew as well as the wild-type parent virus in Vero, baby hamster kidney, and Rat-1 cell lines.

MeSH Terms
Animals Cell Line Chromosome Deletion Cloning, Molecular Genes, Viral Mutation Simplexvirus/genetics,growth & development Viral Proteins/analysis,genetics
Chemicals
Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mavromara-Nazos P
Ackermann M
Roizman B
References (34)
34 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.60.2.807-812.1986
Published
1986-11-00
Pages
807-12
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC288963
Subset
IM
Grants
NCI NIH HHS · CA-08494 · United States
NCI NIH HHS · CA-19264 · United States
External Links
PubMed source DOI: 10.1128/JVI.60.2.807-812.1986 PMC Full Text View journal details
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