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PMID: 3023850 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Extramitochondrial citrate synthase activity in bakers' yeast.

Molecular and cellular biology ·Vol. 6 ·No. 2 ·1986-02-00 ·Pages 488-93

Rickey TM, Lewin AS

Abstract

We isolated the gene for citrate synthase (citrate oxaloacetate lyase; EC 4.1.3.7) from Saccharomyces cerevisiae and ablated it by inserting the yeast LEU2 gene within its reading frame. This revealed a second, nonmitochondrial citrate synthase. Like the mitochondrial enzyme, this enzyme was sensitive to glucose repression. It did not react with antibodies against mitochondrial citrate synthase. Haploid cells lacking a gene for mitochondrial citrate synthase grew somewhat slower than wild-type yeast cells, but exhibited no auxotrophic growth requirements.

MeSH Terms
Citrate (si)-Synthase/genetics DNA Restriction Enzymes Genes Genes, Fungal Mitochondria/enzymology Nucleic Acid Hybridization Oxo-Acid-Lyases/genetics Saccharomyces cerevisiae/enzymology,genetics,growth & development
Chemicals
Citrate (si)-Synthase DNA Restriction Enzymes Oxo-Acid-Lyases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rickey T M
Lewin A S
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.2.488-493.1986
Published
1986-02-00
Pages
488-93
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367537
Subset
IM
Grants
NIGMS NIH HHS · 2R01 GM29387-04A1 · United States
NCRR NIH HHS · S07 RR7031G · United States
External Links
PubMed source DOI: 10.1128/mcb.6.2.488-493.1986 PMC Full Text View journal details
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