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PMID: 3023919 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Multiple regulation of STE2, a mating-type-specific gene of Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 6 ·No. 6 ·1986-06-00 ·Pages 2106-14

Hartig A, Holly J, Saari G, MacKay VL

Abstract

The Saccharomyces cerevisiae STE2 gene, which is required for pheromone response and conjugation specifically in mating-type a cells, was cloned by complementation of the ste2 mutation. Transcription of STE2 is repressed by the MAT alpha 2 gene product, so that the 1.4-kilobase STE2 RNA is detected only in a or mat alpha 2 strains, not in alpha or a/alpha cells. However, STE2 RNA levels are also increased by the mating pheromone alpha-factor and decreased in strains bearing mutations in the nonspecific STE4 gene. Regulation of STE2 expression in a cells is therefore achieved by several mechanisms.

MeSH Terms
Base Sequence Conjugation, Genetic Gene Expression Regulation Genes, Fungal Genes, Mating Type, Fungal Genetic Complementation Test Mating Factor Peptides/physiology Pheromones/physiology Receptors, Cell Surface/genetics Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
Peptides Pheromones Receptors, Cell Surface Mating Factor
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hartig A
Holly J
Saari G
MacKay V L
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.6.2106-2114.1986
Published
1986-06-00
Pages
2106-14
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367751
Subset
IM
Databases
GENBANK
M13481
External Links
PubMed source DOI: 10.1128/mcb.6.6.2106-2114.1986 PMC Full Text View journal details
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