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PMID: 3023920 Published · ppublish English Journal Article

Inverted duplication-transposition event in mammalian cells at an illegitimate recombination join.

Molecular and cellular biology ·Vol. 6 ·No. 6 ·1986-06-00 ·Pages 2179-84

Williams TJ, Fried M

Abstract

Illegitimate recombination events in mammalian cells often contain extraneous nucleotides or filler DNA at the recombinant joins. The polyomavirus-transformed cell line 7axB has previously been found to contain 37 base pairs (bp) of filler DNA at one virus-host join of the single insert of integrated viral DNA (A. Hayday, H. E. Ruley, and M. Fried, J. Virol. 44:67-77, 1982). By using a synthetic oligomer of these 37 bp as a probe, we demonstrated that this filler DNA is an inverted duplication of a single-copy rat sequence found 650 bp upstream from this virus-host join. The other virus-host join appears to be the result of a simple illegitimate recombination event between viral and host sequences. This is the first identification of filler DNA as a transposed copy of a chromosomal sequence. The relevance of the recombination events studied to cellular rearrangements and viral integration is discussed.

MeSH Terms
Animals Base Sequence Polyomavirus/genetics Rats Recombination, Genetic
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Williams T J
Fried M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.6.2179-2184.1986
Published
1986-06-00
Pages
2179-84
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367758
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.6.6.2179-2184.1986 PMC Full Text View journal details
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