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PMID: 3024129 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cloning of cDNA for human T-cell replacing factor (interleukin-5) and comparison with the murine homologue.

Nucleic acids research ·Vol. 14 ·No. 22 ·1986-11-25 ·Pages 9149-58

Azuma C, Tanabe T, Konishi M, Kinashi T, Noma T, Matsuda F, Yaoita Y, Takatsu K, Hammarström L, Smith CI

Abstract

We have cloned cDNA for T-cell replacing factor (interleukin-5), which replaces T-cell helper function for normal B cells which secrete immunoglobulin, from human T cell leukemia line, ATL-2, using mouse interleukin-5 cDNA as probe. Total nucleotide sequence of the cDNA (816 base pairs) was determined and compared with that of mouse interleukin-5 cDNA. The cloned cDNA encoded the interleukin-5 precursor of 134 amino acids containing an N-terminal signal sequence. Although the human interleukin-5 precursor is one amino acid longer than the murine homologue, the sizes of the mature proteins appear similar. The nucleotide and amino acid sequence homologies of the coding regions of human and murine interleukin-5 are 77% and 70%, respectively. Human interleukin-5 synthesized by the direction of the cloned cDNA induced immunoglobulin synthesis in human B cells stimulated by Staphylococcus aureus mitogen.

MeSH Terms

Amino Acid Sequence Animals Cell Line Cloning, Molecular DNA/metabolism DNA Restriction Enzymes Humans Interleukin-5 Lymphokines/genetics Mice Sequence Homology, Nucleic Acid T-Lymphocytes/immunology

Chemicals

Interleukin-5 Lymphokines DNA DNA Restriction Enzymes

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Azuma C

Tanabe T

Konishi M

Kinashi T

Noma T

Matsuda F

Yaoita Y

Takatsu K

Hammarström L

Smith C I

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/14.22.9149

Published

1986-11-25

Pages

9149-58

Language

English

Region

England

NLM ID

0411011

PMCID

PMC311935

Subset

Databases

GENBANK

X04688

External Links

PubMed source DOI: 10.1093/nar/14.22.9149 PMC Full Text View journal details

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Cloning of cDNA for human T-cell replacing factor (interleukin-5) and comparison with the murine homologue.

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