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PMID: 3025586 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sites of P element insertion and structures of P element deletions in the 5' region of Drosophila melanogaster RpII215.

Molecular and cellular biology ·Vol. 6 ·No. 10 ·1986-10-00 ·Pages 3312-9

Searles LL, Greenleaf AL, Kemp WE, Voelker RA

Abstract

Several P element insertion and deletion mutations near the 5' end of Drosophila melanogaster RpII215 have been examined by nucleotide sequencing. Two different sites of P element insertion, approximately 90 nucleotides apart, have been detected in this region of the gene. Therefore, including an additional site of P element insertion within the coding region, there are at least three distinct sites of P element insertion at RpII215. Both 5' sites are within a noncoding portion of transcribed sequences. The sequences of four revertants of one P element insertion mutation (D50) indicate that the P element is either precisely deleted or internally deleted to restore RpII215 activity. Partial internal deletions of the P element result in different RpII215 activity levels, which appear to depend on the specific sequences that remain after excision.

MeSH Terms
Animals Base Sequence Chromosome Deletion Cloning, Molecular DNA Restriction Enzymes DNA Transposable Elements Drosophila melanogaster/genetics Genes Mutation
Chemicals
DNA Transposable Elements DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Searles L L
Greenleaf A L
Kemp W E
Voelker R A
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32 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.10.3312-3319.1986
Published
1986-10-00
Pages
3312-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367076
Subset
IM
Databases
GENBANK
M14203, M14204, M14205, M20239, M20240
External Links
PubMed source DOI: 10.1128/mcb.6.10.3312-3319.1986 PMC Full Text View journal details
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