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PMID: 3025627 Published · ppublish English Journal Article

A nuclear gene of Saccharomyces cerevisiae needed for stable maintenance of plasmids.

Molecular and cellular biology ·Vol. 6 ·No. 11 ·1986-11-00 ·Pages 4053-9

Kikuchi Y, Toh-e A

Abstract

We have isolated host mutants of Saccharomyces cerevisiae in which the 2 microns plasmid is poorly maintained. All the mutants tested constituted one complementation group, which was designated map1 (maintenance of plasmid). Minichromosomes carrying a chromosomal replication origin and a centromere were affected in the mutants. Two types of hybrid plasmids generated in vivo and in vitro appeared to compensate for the mutations and had DNA regions containing multiple ARS (autonomously replicating sequence) or a set of 2 microns inverted repeat sequences. These results suggested that poor maintenance of plasmids was due to low levels of replication, probably at the initiation of replication.

MeSH Terms
Cell Nucleus/physiology Crosses, Genetic DNA Restriction Enzymes Genes, Fungal Genotype Mutation Plasmids Saccharomyces cerevisiae/genetics
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kikuchi Y
Toh-e A
References (41)
41 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.11.4053-4059.1986
Published
1986-11-00
Pages
4053-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367171
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.6.11.4053-4059.1986 PMC Full Text View journal details
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