Home LiteratureArticle Details
PMID: 3025641 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Binding in vitro of multiple cellular proteins to immunoglobulin heavy-chain enhancer DNA.

Molecular and cellular biology ·Vol. 6 ·No. 12 ·1986-12-00 ·Pages 4168-78

Peterson CL, Orth K, Calame KL

Abstract

Seven protein-binding sites on the immunoglobulin heavy-chain (IgH) enhancer element have been identified by exonuclease III protection and gel retardation assays. It appears that the seven sites bind a minimum of four separate proteins. Three of these proteins also bind to other enhancers or promoters, but one protein seems to recognize exclusively IgH enhancer sequences. A complex of four binding sites, recognized by different proteins, is located within one 80-base-pair region of IgH enhancer DNA. Close juxtaposition of enhancer proteins may allow protein-protein interactions or be part of a mechanism for modulating enhancer protein activity. All IgH enhancer-binding proteins identified in this study were found in extracts from nonlymphoid as well as lymphoid cells. These data provide the first direct evidence that multiple proteins bind to enhancer elements and that while some of these proteins recognize common elements of many enhancers, others have more limited specificities.

MeSH Terms
Animals Base Sequence Binding Sites Cell Line DNA Restriction Enzymes DNA-Binding Proteins/metabolism Enhancer Elements, Genetic Genes, Regulator Immunoglobulin Heavy Chains/genetics Mice Neoplasm Proteins/metabolism Plasmacytoma/immunology Protein Binding
Chemicals
DNA-Binding Proteins Immunoglobulin Heavy Chains Neoplasm Proteins DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Peterson C L
Orth K
Calame K L
References (50)
50 references, click to expand
  1. A trans-acting factor is responsible for the simian virus 40 enhancer activity in vitro.
    Nature. 1985 Feb 7-13;313(6002):458-63 PMID: 2982105
  2. DNA sequences bound specifically by glucocorticoid receptor in vitro render a heterologous promoter hormone responsive in vivo.
    Cell. 1983 Jun;33(2):489-99 PMID: 6190571
  3. A 46-nucleotide promoter segment from an IFN-alpha gene renders an unrelated promoter inducible by virus.
    Cell. 1985 Jun;41(2):497-507 PMID: 3986909
  4. Human beta-interferon gene expression is regulated by an inducible enhancer element.
    Cell. 1985 Jun;41(2):509-20 PMID: 3986910
  5. Simian virus 40 enhancer increases number of RNA polymerase II molecules on linked DNA.
    Nature. 1985 May 2-8;315(6014):73-5 PMID: 2986013
  6. Simian virus 40 enhancer increases RNA polymerase density within the linked gene.
    Nature. 1985 May 2-8;315(6014):75-7 PMID: 2986014
  7. Two distinct transcription factors bind to the HSV thymidine kinase promoter in vitro.
    Cell. 1985 Sep;42(2):559-72 PMID: 2992804
  8. An exonuclease protection assay reveals heat-shock element and TATA box DNA-binding proteins in crude nuclear extracts.
    Nature. 1985 Sep 5-11;317(6032):84-7 PMID: 2993917
  9. Expression and regulation of immunoglobulin heavy chain gene transfected into lymphoid cells.
    EMBO J. 1983;2(8):1373-8 PMID: 10872333
  10. Three IgA myeloma immunoglobulins from the BALB/ mouse: precipitation with pneumococcal C polysaccharide.
    Science. 1968 Oct 18;162(3851):369-71 PMID: 4175691
  11. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  12. Exonuclease III of Escherichia coli K-12, an AP endonuclease.
    Methods Enzymol. 1980;65(1):201-11 PMID: 6246343
  13. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  14. In vivo sequence requirements of the SV40 early promotor region.
    Nature. 1981 Mar 26;290(5804):304-10 PMID: 6259538
  15. Rearranged and germline immunoglobulin kappa genes: different states of DNase I sensitivity of constant kappa genes in immunocompetent and nonimmune cells.
    Biochemistry. 1981 Feb 17;20(4):990-6 PMID: 6260146
  16. IgG antibodies to phosphorylcholine exhibit more diversity than their IgM counterparts.
    Nature. 1981 May 7;291(5810):29-34 PMID: 7231520
  17. Simian virus 40 tandem repeated sequences as an element of the early promoter.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):943-7 PMID: 6262784
  18. The SV40 72 base repair repeat has a striking effect on gene expression both in SV40 and other chimeric recombinants.
    Nucleic Acids Res. 1981 Nov 25;9(22):6047-68 PMID: 6273820
  19. A small segment of polyoma virus DNA enhances the expression of a cloned beta-globin gene over a distance of 1400 base pairs.
    Nucleic Acids Res. 1981 Dec 11;9(23):6251-64 PMID: 6275354
  20. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
    Nucleic Acids Res. 1981 Dec 11;9(23):6505-25 PMID: 6275366
  21. Host-specific activation of transcription by tandem repeats from simian virus 40 and Moloney murine sarcoma virus.
    Proc Natl Acad Sci U S A. 1982 Nov;79(21):6453-7 PMID: 6292901
  22. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  23. A GAL10-CYC1 hybrid yeast promoter identifies the GAL4 regulatory region as an upstream site.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7410-4 PMID: 6760197
  24. Contact points between a positive transcription factor and the Xenopus 5S RNA gene.
    Cell. 1982 Dec;31(2 Pt 1):395-405 PMID: 6297765
  25. The SV40 72 bp repeat preferentially potentiates transcription starting from proximal natural or substitute promoter elements.
    Cell. 1983 Feb;32(2):503-14 PMID: 6297796
  26. Enhancer elements.
    Cell. 1983 Jun;33(2):313-4 PMID: 6305503
  27. Transcriptional enhancer elements in the mouse immunoglobulin heavy chain locus.
    Science. 1983 Aug 12;221(4611):663-5 PMID: 6306772
  28. A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene.
    Cell. 1983 Jul;33(3):717-28 PMID: 6409417
  29. A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes.
    Cell. 1983 Jul;33(3):729-40 PMID: 6409418
  30. Immunoglobulin gene transcription is activated by downstream sequence elements.
    Cell. 1983 Jul;33(3):741-8 PMID: 6409419
  31. DNase I hypersensitive sites in the chromatin of human mu immunoglobulin heavy-chain genes.
    Nature. 1983 Dec 22-1984 Jan 4;306(5945):809-12 PMID: 6419125
  32. Specific interaction between enhancer-containing molecules and cellular components.
    Cell. 1984 Feb;36(2):403-11 PMID: 6319021
  33. Characterization of DNA sequences through which cadmium and glucocorticoid hormones induce human metallothionein-IIA gene.
    Nature. 1984 Apr 5-11;308(5959):513-9 PMID: 6323998
  34. Structure of the 5' ends of immunoglobulin genes: a novel conserved sequence.
    Proc Natl Acad Sci U S A. 1984 May;81(9):2650-4 PMID: 6425835
  35. A Drosophila RNA polymerase II transcription factor binds to the regulatory site of an hsp 70 gene.
    Cell. 1984 May;37(1):273-83 PMID: 6722872
  36. Correct transcription of an immunoglobulin kappa gene requires an upstream fragment containing conserved sequence elements.
    Nature. 1984 Jul 5-11;310(5972):71-4 PMID: 6330567
  37. Multiple specific contacts between a mammalian transcription factor and its cognate promoters.
    Nature. 1984 Nov 29-Dec 5;312(5993):409-13 PMID: 6095100
  38. B lineage--specific interactions of an immunoglobulin enhancer with cellular factors in vivo.
    Science. 1985 Jan 11;227(4683):134-40 PMID: 3917574
  39. Immunoglobulin heavy-chain enhancer requires one or more tissue-specific factors.
    Science. 1985 Jan 18;227(4684):266-70 PMID: 3917575
  40. A positive transcription factor controls the differential expression of two 5S RNA genes.
    Cell. 1985 Oct;42(3):759-67 PMID: 4053184
  41. Cell-specific expression of the rat insulin gene: evidence for role of two distinct 5' flanking elements.
    Science. 1985 Nov 22;230(4728):912-6 PMID: 3904002
  42. Cell type-specific transcriptional enhancement in vitro requires the presence of trans-acting factors.
    EMBO J. 1985 Nov;4(11):3005-13 PMID: 2998773
  43. Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region.
    Cell. 1985 Nov;43(1):165-75 PMID: 4075392
  44. A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes.
    Nature. 1986 Jan 9-15;319(6049):154-8 PMID: 3079885
  45. A purified transcription factor (TIF-IB) binds to essential sequences of the mouse rDNA promoter.
    Proc Natl Acad Sci U S A. 1986 Feb;83(3):604-8 PMID: 3456157
  46. In vivo competition between a metallothionein regulatory element and the SV40 enhancer.
    Science. 1986 Apr 4;232(4746):76-80 PMID: 3006253
  47. Nuclear activity from F9 embryonal carcinoma cells binding specifically to the enhancers of wild-type polyoma virus and PyEC mutant DNAs.
    Nucleic Acids Res. 1986 Apr 11;14(7):2845-61 PMID: 2421253
  48. Human rRNA transcription is modulated by the coordinate binding of two factors to an upstream control element.
    Cell. 1986 Jun 20;45(6):847-57 PMID: 3708692
  49. Binding of Drosophila heat-shock gene transcription factor to the hsp 70 promoter. Evidence for symmetric and dynamic interactions.
    J Biol Chem. 1986 Jun 15;261(17):7934-40 PMID: 3711119
  50. Specific DNA binding of GAL4, a positive regulatory protein of yeast.
    Cell. 1985 Apr;40(4):767-74 PMID: 3886158
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.12.4168-4178.1986
Published
1986-12-00
Pages
4168-78
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367196
Subset
IM
Grants
NCI NIH HHS · CA38571 · United States
NIGMS NIH HHS · GM 07185 · United States
NIGMS NIH HHS · GM 29361 · United States
External Links
PubMed source DOI: 10.1128/mcb.6.12.4168-4178.1986 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2