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PMID: 3026801 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of an isogenic set of methicillin-resistant and susceptible mutants of Staphylococcus aureus.

European journal of clinical microbiology ·Vol. 5 ·No. 6 ·1986-12-00 ·Pages 697-701

Berger-Bächi B, Strässle A, Kayser FH

Abstract

A low affinity penicillin binding protein (PBP2') present in methicillin resistant strains but absent in isogenic sensitive strains of Staphylococcus aureus is responsible for the phenotypic expression of the methicillin resistance. An additional factor controlling methicillin resistance is known to map on the chromosome at the insertion site omega 2003 (chr::Tn551). The influence of this factor on PBP2' synthesis was analysed. Isogenic methicillin sensitive and resistant mutants derived from a Staphylococcus aureus strain carrying methicillin resistance factors and the omega 2003 (chr::Tn551) insertion were characterized by population analysis and restriction analysis of the DNA surrounding the Tn551 insertion site, and their PBP2' content was determined. Both methicillin resistant and sensitive mutants produced levels of PBP2' similar to those of the original methicillin resistant parent. The mere presence of this PBP2' could therefore not be solely responsible for the phenotypic expression of methicillin resistance. The nature and site of action of the additional factor determined by the omega 2003 (chr::Tn551) site controlling expression of methicillin resistance still remains unknown.

MeSH Terms
Bacterial Proteins Carrier Proteins/biosynthesis,genetics DNA Transposable Elements DNA, Bacterial/analysis Hexosyltransferases Methicillin/pharmacology Muramoylpentapeptide Carboxypeptidase/biosynthesis,genetics Mutation Nucleic Acid Hybridization Penicillin Resistance Penicillin-Binding Proteins Peptidyl Transferases Phenotype R Factors Staphylococcus aureus/drug effects,genetics,metabolism
Chemicals
Bacterial Proteins Carrier Proteins DNA Transposable Elements DNA, Bacterial Penicillin-Binding Proteins Peptidyl Transferases Hexosyltransferases Muramoylpentapeptide Carboxypeptidase Methicillin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Berger-Bächi B
Strässle A
Kayser F H
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Article Info
Journal
European journal of clinical microbiology
Abbr.
Eur J Clin Microbiol
ISSN
0722-2211
DOI
10.1007/BF02013308
Published
1986-12-00
Pages
697-701
Language
English
Region
Germany
NLM ID
8219582
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02013308
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