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PMID: 3027121 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Myosin specific phosphatases isolated from Dictyostelium discoideum.

Journal of muscle research and cell motility ·Vol. 7 ·No. 6 ·1986-12-00 ·Pages 510-6

Kuczmarski ER, Pagone J

Abstract

Using native myosin phosphorylated on either the heavy chain or the light chain, we have isolated myosin phosphatases from extracts of vegetative Dictyostelium amoeba. Two phosphatases were resolved by DEAE-cellulose chromatography. One of these phosphatases removed phosphate from heavy chain or light chain at approximately the same rate. The other phosphatase appeared to be much more specific for phosphorylated myosin heavy chain. Although these enzymes removed phosphate from other phosphoprotein substrates such as histone or casein, they did so at a much lower rate. Both enzymes required magnesium for activity, but appeared to be independent of calcium.

MeSH Terms
Animals Calcium/pharmacology Dictyostelium/enzymology Magnesium/pharmacology Myosin-Light-Chain Phosphatase Myosins/metabolism Phosphoprotein Phosphatases/isolation & purification Phosphorylation Substrate Specificity
Chemicals
Phosphoprotein Phosphatases Myosin-Light-Chain Phosphatase Myosins Magnesium Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kuczmarski E R
Pagone J
References (25)
25 references, click to expand
  1. Localization of two phosphorylation sites adjacent to a region important for polymerization on the tail of Dictyostelium myosin.
    EMBO J. 1984 Dec 20;3(13):3271-8 PMID: 16453592
  2. Dictyostelium discoideum: methods and perspectives for study of cell motility.
    Methods Cell Biol. 1982;25 Pt B:359-64 PMID: 7109964
  3. Purification of a myosin phosphatase from bovine aortic smooth muscle.
    J Biol Chem. 1982 Jul 10;257(13):7306-9 PMID: 6282839
  4. Purification and characterization of a myosin I heavy chain kinase from Acanthamoeba castellanii.
    J Biol Chem. 1983 Aug 25;258(16):10168-75 PMID: 6309772
  5. Reversible cyclic AMP-dependent change in distribution of myosin thick filaments in Dictyostelium.
    Nature. 1985 Mar 14-20;314(6007):194-6 PMID: 2983234
  6. Calcium control of actin-activated myosin adenosine triphosphatase from Dictyostelium discoideum.
    Proc Natl Acad Sci U S A. 1976 Jul;73(7):2321-5 PMID: 133352
  7. BHK21 myosin: isolation, biochemical characterization and intracellular localization.
    J Cell Sci. 1978 Jun;31:411-29 PMID: 149798
  8. Actin activation of Ca2+-sensitive Mg2+-ATPase activity of Acanthamoeba myosin II is enhanced by dephosphorylation of its heavy chains.
    J Biol Chem. 1980 Sep 10;255(17):8011-4 PMID: 6106017
  9. Cyclic AMP-induced phosphorylation in Dictyostelium of a polypeptide comigrating with myosin heavy chains.
    FEBS Lett. 1978 Apr 15;88(2):322-6 PMID: 206468
  10. Roles of calcium and phosphorylation in the regulation of the activity of gizzard myosin.
    Biochemistry. 1978 Oct 17;17 (21):4411-8 PMID: 152641
  11. Dictyostelium myosin: characterization of chymotryptic fragments and localization of the heavy-chain phosphorylation site.
    J Cell Biol. 1981 Apr;89(1):104-8 PMID: 7228895
  12. A phosphorylated light-chain component of myosin from skeletal muscle.
    Biochem J. 1973 Sep;135(1):151-64 PMID: 4776866
  13. Ligand-induced changes in the location of actin, myosin, 95K (alpha-actinin), and 120K protein in amebae of Dictyostelium discoideum.
    J Cell Biol. 1985 Jun;100(6):1884-93 PMID: 3889011
  14. Purification and characterization of a multisubunit phosphatase from turkey gizzard smooth muscle. The effect of calmodulin binding to myosin light chain kinase on dephosphorylation.
    J Biol Chem. 1983 Jun 10;258(11):7047-54 PMID: 6304072
  15. Chemoattractant-elicited increases in myosin phosphorylation in Dictyostelium.
    Cell. 1985 Nov;43(1):307-14 PMID: 3000604
  16. Purification of a protein phosphatase from Acanthamoeba that dephosphorylates and activates myosin II.
    J Biol Chem. 1983 Dec 10;258(23):14570-5 PMID: 6315729
  17. Isolation of concanavalin A caps during various stages of formation and their association with actin and myosin.
    J Cell Biol. 1979 Mar;80(3):751-8 PMID: 457767
  18. Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein.
    Anal Biochem. 1981 Sep 1;116(1):53-64 PMID: 7304986
  19. Regulation of myosin self-assembly: phosphorylation of Dictyostelium heavy chain inhibits formation of thick filaments.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7292-6 PMID: 6452632
  20. Purification and characterization of actin-activatable, Ca2+-sensitive myosin II from Acanthamoeba.
    J Biol Chem. 1981 Mar 10;256(5):2586-95 PMID: 6109730
  21. Phosphorylation of platelet myosin increases actin-activated myosin ATPase activity.
    Nature. 1975 Aug 14;256(5518):597-8 PMID: 170529
  22. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  23. Myosin light-chain phosphatase.
    Biochem J. 1976 Sep 1;157(3):687-97 PMID: 186030
  24. Biochemical and structural studies of actomyosin-like proteins from non-muscle cells. Isolation and characterization of myosin from amoebae of Dictyostelium discoideum.
    J Mol Biol. 1974 Jun 25;86(2):209-22 PMID: 4278009
  25. Partial purification of two myosin heavy chain kinases from Dictyostelium discoideum.
    J Muscle Res Cell Motil. 1986 Dec;7(6):501-9 PMID: 3027120
Article Info
Journal
Journal of muscle research and cell motility
Abbr.
J Muscle Res Cell Motil
ISSN
0142-4319
DOI
10.1007/BF01753567
Published
1986-12-00
Pages
510-6
Language
English
Region
Netherlands
NLM ID
8006298
Subset
IM
Grants
NIGMS NIH HHS · GM 31907 · United States
External Links
PubMed source DOI: 10.1007/BF01753567 View journal details
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